摘要
利用抗Rb基因蛋白产物的多克隆抗体对15例视网膜母细胞瘤Rb基因表达水平进行Western印迹分析,并采用激光密度分析仪对结果进行进一步精确的定量分析,表明15例视网膜母细胞瘤中仅有两例的Rb基因蛋白表达量与正常视网膜相似,其余13例几乎没有Rb基因的表达产物。提示在大多数视网膜母细胞瘤中,Rb基因的蛋白质产物缺乏。
With the anti-Rb polyclonal antibody,the expression of Rb gene in 15 cases of retinoblastoma were studied by Western Bloting.Using laser densitometry technique,we made a quantitative comparison of the expression dose of Rb gene product between Rb tumor and normal retinal tissues.The relative concentrations of p105Rb in 13 RB patients were remarkably lower than those of normals. The results suggest that Rb gene activity is possibly decreased in most cases of RB.
出处
《中华医学遗传学杂志》
CAS
CSCD
北大核心
1994年第1期16-18,共3页
Chinese Journal of Medical Genetics
基金
国家重点实验室资助