视网膜母细胞瘤Rb基因表达

THE EXPRESSION OF Rb GENE IN RETINOBLASTOMA

利用抗Ｒｂ基因蛋白产物的多克隆抗体对１５例视网膜母细胞瘤Ｒｂ基因表达水平进行Ｗｅｓｔｅｒｎ印迹分析，并采用激光密度分析仪对结果进行进一步精确的定量分析，表明１５例视网膜母细胞瘤中仅有两例的Ｒｂ基因蛋白表达量与正常视网膜相似，其余１３例几乎没有Ｒｂ基因的表达产物。提示在大多数视网膜母细胞瘤中，Ｒｂ基因的蛋白质产物缺乏。

With the anti-Rb polyclonal antibody,the expression of Rb gene in 15 cases of retinoblastoma were studied by Western Bloting.Using laser densitometry technique,we made a quantitative comparison of the expression dose of Rb gene product between Rb tumor and normal retinal tissues.The relative concentrations of p105Rb in 13 RB patients were remarkably lower than those of normals. The results suggest that Rb gene activity is possibly decreased in most cases of RB.