初诊和复发急性髓性白血病p15、p16基因甲基化研究

Study of the methylation of p15 and p16 gene in acute leukemia

目的探讨急性髓性白血病(AL)p15、p16、p18、p19基因失活的发生率及与疾病发生、发展、预后的关系。方法用聚合酶链反应(PCR)方法扩增46例患者的p15、p16、p18、p19基因外显子1和外显子2,再用限制性内切酶-PCR方法检测基因甲基化。结果46例患者中,急性淋巴细胞白血病(ALL)19例,11例p15基因失活,10例p16基因失活;急性非淋巴细胞白血病(ANLL)27例,9例p15基因失活,18例p16基因失活;均以甲基化失活为主。所有病例均无p18、p19基因失活。结论在AL发生、发展过程中,p15、p16基因失活主要是由于p15、p16基因甲基化所致。

Objective To investigate the inactived frequency of p15 and p16 gene in acute leukemia and to evaluate its clinical significance and explore the role of hypermethylation of p15 and p16 gene in the pathogenesis of acute leukemia.Methods There were 46 cases of acute leukemia which included 19 ALL cases and 27 ANLL cases.Using PCR technique to detect homozygous dilation of p15 and p16 gene,restriction enzyme-PCR technique was used to detect gene methylation.Results There were 11 inactived cases of p15 and 10 inactived cases of p16 in 19 ALL patients.There were 9 inactived cases of p15 and 18 inactived cases of p16 in 27 ANLL patients.The methylation was a major way of inactivation of p15 and p16 gene.The positive rate of MSP results was 74% in ALL and 82% in ANLL.Conclusion The inactivation of p15 and p16 gene plays a key role in the pathogenesis of acute leukemia which methylation of p15 and p16 gene are the major way of gene inactivation.