二硫化碳诱发人精子染色体畸变

THE EFFECTS OF CARBON DISULPHIDE ON THE INDUCTION OF CHROMOSOMAL ABERRATION IN HUMAN SPERMATOZOA

采用人精子染色体离体测试系统，研究二硫化碳（ＣＳ２）对人精子染色体诱变作用。ＣＳ２１μｍｏｌ／Ｌ、５μｍｏｌ／Ｌ、１０μｍｏｌ／ｍｌ各剂量组结构畸变率分别为４．０％、１２．０％、３５．７％。断裂均数分别为０．０４０、０．１２０、０．６４３，与对照组畸变精子率４．２％和断裂均数０．０４２比较，１０μｍｏｌ／Ｌ组与对照组间的差异具有统计学意义（Ｐ＜０．００１）。提示ＣＳ２在达到一定剂量时可直接作用于精细胞，使精细胞遗传物质受损。

The effects of carbon disulphide(CS2) on the induction of chromosome abnormalities in human spermatozoa were studied by means of in vitro tes system of human sperm chromosome. The sperm samples were exposed to CS2 at the concentrations of 1, 5, 10μmol/ml for 3.5 hours respectively during the period of their preincubation before interspecific in vitro fertilization with zona-free hamster oocytes.The mean of chromosome breakages were 0.040,0.120,0. 643 per spermatozoon sequentially in the above dosage groups. Both the incidence of spermatozoa with structurally chromosomal aberrations and the mean of breakages in the CS2(10μmol/L)-exposed group were statistically higher than those in the control(P<0. 001).The results suggested that the mutagenic effects on male gametes would be induced by certain amount of CS2.