二磷酸果糖对老年大鼠缺血性急性肾功能衰竭的防护作用

Protcctive effcct of 1,6-diphosphate fructose inischemic renal failure in eldcrly rats

缺血／再灌引起细胞线粒体功能明显减退，三磷酸腺苷（ＡＴＰ）合成减少，是导致急性肾功能衰竭（ＡＲＦ）的重要因素之一。老年肾对缺血／再灌损伤敏感，易发生ＡＲＦ，且预后差。已知１，６－二磷酸果糖（ＦＤＰ）对心、脑缺血损伤有保护作用。我们通过观察ＦＤＰ对不同年龄组缺血性ＡＲＦ大鼠的肾功能、线粒体功能的影响，发现ＦＤＰ对其肾功能（菊糖清除率ＣＩｎ）和线粒体功能（ＡＴＰ合成量）确有保护作用，且对老年大鼠的防护作用优于对青年大鼠的作用。提示ＦＤＰ对老年肾脏缺血性损伤有特殊意义。

o study the protective effect of 1 , 6-diphosphatefructose (FDP) on acute ischemia damage in brain,heart and liver, we observed its effect on acuteischemic renal failure (fAIRF). AIRF in male Wisterrats was produced by renal arterial clamping for 45minutes and reperfusion for 90 minutes. The rats weredivided into 4 groups according to their age and FDPtherapy. G1 : young (3- 4 months) AIRF rats withoutFDP, G2: old (26- 27 months) AIRF rats withoutFDP; G3: young AIRF rats with FDP (0.59 / kg) ivinfusion; G4: old AIRF rats with FDP. Inulin clear-ance (CIn), PAH clearance (CPAH), urinary sodium(UNA) were examined before ischemia and 30, 60 and90 minutes after reperfusion. After reperfusion for 90minutes, renal cortex was taken for the examination ofrenal mitochondrial ATP synthetic content (miATPs).CIn and CPAH were lower in G2 than in G1 (0.24 ± 0. 12vs 1.75± 0.79, P<0.01, 0.87± 0.42 vs 7.12± 4.04,P<0.05, at 90 minutes reperfusion). C,.and CPAH inG1 showed an autorecovery up to 40.1% and 33.7%of the level in contrast to G2. In G3 and G4 originalFDP infusion resulted in a significant increase inmiATPs and CIn(609 ± 145 vs 389 ± 97, 569 ± 77 vs 338± 61, P < 0.02, 2.47 ± 0.58 VS 1 .75 ± 0.79, 1 .29 ± 0.42 vs0.24 ± 0. 12, P < 0.01). CIn of the old was higher thanthat of the young and the miATPs increased more evi-dently in the old than in the young (6.0 vs 3.5 times,and 68.% vs 57%). These data suggest that FDPbnngs about greater protective effect on the old thanon the young from AIRF, which might relate to itsrole of energe providing under anaerobic glycolysiscondition.