摘要
喹乙醇、阿散酸和土霉素是应用极广的兽药添加剂,在畜牧业的发展中起着特殊的作用,但其使用不当或过量使用会给环境带来很大危害.采用土壤中的重要生物赤子爱胜蚓作为生物材料,分别研究了喹乙醇、阿散酸和土霉素对赤子爱胜蚓的急性毒性和对赤子爱胜蚓体腔细胞DNA的损伤.结果表明,喹乙醇溶液法LC50>2000mg·L-1,滤纸法LC50>5.71×10-2mg·cm-2,而阿散酸和土霉素在所设最大受试剂量内没有毒性.单细胞凝胶电泳试验结果表明,阿散酸没有引起蚯蚓体腔细胞DNA明显损伤;喹乙醇和土霉素均能引起一定程度的DNA损伤,中、高剂量组与阴性对照相比有极显著差异(P<0.01).因此,有必要加强对以上兽药添加剂,特别是喹乙醇和土霉素的管理,控制用药时间和剂量,以确保人体健康和环境安全.
By the methods of acute toxicity test and single cell gel electrophoresis (comet assay),this paper evaluated the toxicological effects of three veterinary drugs olaquindox,arsanilic acid and oxytetracycline on earthworm (Eisenia foetida) coelomocytes in vivo.The results of acute toxicity test showed that only the highest dose of olaquidox caused the death of some earthworms,and none of the test drugs had any effects on earthworm at their environmentally relevant concentrations.The comet assay indicated that arsanilic acid had no genotoxicity to earthworm,while olaquindox and oxytetracycline induced significant DNA damage in earthworm coelomocytes (P<0.01).
出处
《应用生态学报》
CAS
CSCD
北大核心
2005年第6期1108-1111,共4页
Chinese Journal of Applied Ecology
基金
高等学校博士学科点专项科研基金资助项目(20020284041).
关键词
兽药添加剂
喹乙醇
阿散酸
土霉素
赤子爱胜蚓
急性毒性试验
单细胞凝胶电泳
Veterinary additives, Olaquindox, Arsanilic acid, Oxytetracycline, Eisenia foetida, Acute toxicity test, Single cell gel electrophoresis test(SCGE).
