胰腺癌组织中K－ras基因点突变的研究

Point mutation of K-ras gene in pancreatic carcinoma

应用Ｔａｑ酶直接测序技术对９例胰腺癌标本及１例恶性胰岛细胞瘤标本进行了Ｋ－ｒａｓ基因１２位点突变的检测。结果显示：９例胰腺癌中含Ｋ－ｒａｓ基因突变者５例。表现出２种突变类型，即ＣＣＡ→ＣＧＡ型４例；ＣＣＡ→ＣＡＡ型１例。相应氨基酸改变分别为：甘氨酸→丙氨酸；甘氨酸→缬氨酸。分析了在突变率及突变内容，上与国外学者不同的原因，探讨了检测Ｋ－ｒａｓ基因１２位点突变的意义。

irect sequencing using Taq enzyme was estab-lished for determination of point mutation of K-rasgene at codon 12 in 9 wax samples of pancreaticcarcincma (PC) and 1 of islet cell tumor. Point muta-tion occurred in 5 of 9 samples of PC and manifestedtwo types of mutation, CCA→CGA in 4 and CCA→CAA in l. The changes of amino acid includedchanges of glycine to alanine and glycine to valine.The causes of mutation frequency and the content dif-fered iron that of foreign reports were analysed inaddition to the significance of determining point muta-tion of K-ras gene at codon 12.