摘要
应用兔抗牛丝虫抗体ELISA双抗体夹心法和Dot-ELISA法检测安徽流行区人体班氏丝虫循环抗原。微丝蚴血症者血清总阳性率96%(24/25),尿液总阳性率为60%(15/25),4份乳汁阳性率均为100%。15份尿液阳性和4份乳汁阳性者与血清阳性结果的符合率分别为100%(15/15)和75%(3/4)。检测江苏非流行区正常人血清、尿液、乳汁共43份,全部阴性。11份肠道线虫感染者尿液全为阴性,血清Dot-ELISA阴性。10份肺吸虫感染者血清Dot-ELISA阴性。结果表明,班氏丝虫循环抗原不仅存在于血清而且存在于尿液和乳汁中。
he ELISA and Dot-ELISA with rabbitanti-Setaria labiat-papillosa
antibody were applied todetermine circulating antigens of Filaria bancrofti.The total positive rate
was 96% (24 / 25) in the sera ofpatients with microfilaremia from endemic areas,Anhui province.
The total positive rate of urines was60% (15 / 25) in the same patients. The positive ratesof the
milks were both 100% (4/4) in the same pa-tients. The conformity rate was 100% and
75%respectively for positive sera and unnes (15 cases).milks (4). Negative reactions were
observed in the sera,urines and milks of all the 43 healthy people fromnon-endemic areas in
Jiangsu province. Negative re-actions were observed in the unnes of all the 11non-filaria cases
harbouring intestinal nematodas.The positive rate detected with ELISA was 36%(4 /11) in the
patient sera that were negative whenthey were detected by Dot-ELISA. Negative reactionswere
detected with Dot-ELISA in the sera of all the10 non-filaria patients infected with
Paragonimuswestermani. The results showed that circulatingantigens of Filaria bancrofti exist
not only in the se-rum but also in the urine and the milk.
出处
《中华医学杂志》
CAS
CSCD
北大核心
1994年第3期155-157,共3页
National Medical Journal of China
基金
国家自然科学基金
关键词
丝虫病
抗原
尿液
乳液
Filariasis Antigen UrineMilk,
humam