^(131)I标记抗CEA单抗在荷人乳腺癌裸鼠体内的放射免疫显像及生物学分布

Radioimmunoimaging and biodistribution of ^(131)I labeled anti-CEA monoclonal Antibody C50 in nude mice bearing human breast carcinoma xenografts

目的研究131I标记的抗CEA单克隆抗体C50(简称131I-C50)及正常小鼠IgG(简称131I-mIgG)在荷人乳腺癌裸鼠体内的放射免疫显像及生物学分布,为临床应用提供依据。方法每只荷瘤鼠尾静脉注射标记抗体3·7MBq/0·1ml,于注射后24、48、96、120小时行SPECT,于48、96、120小时分批处死,测定肿瘤、血液、肝、肺、等重要脏器的单位重量放射性比值(T/NT)及各组织摄取百分比(%ID/g)。结果131I-C50注射后24~120小时内,SPECT可见阳性显像,阳性率为50%;瘤/血比值在120小时最高,达3·07±1·01。131I-mIgG注射后在24~120小时内未出现放射性浓聚呈全身性分布。结论C50在荷人乳腺癌裸鼠体内,对过度表达CEA的乳腺癌具有亲合力,可望用作乳腺癌诊断及预后判断的导向载体。

Objective To study the Radioimmunoimaging and distribution of ^(131)I labeled anti-CEA monoclonal antibody(McAb) C50 and normal mice IgG in made mice bearing human breast Carcinoma and to provide the basis for clinical use. Methods 3.7 MBq/0.1 ml labeled antibody was injected into the tail vein in each respectively SPECT were measured after 24th 48th 96th 120th hours interval of injection; Radioactivity ratio of tumor end non-tumor(T/NT), Percent injected dosage of every gram tissue(%ID/g) were measured after 48th, 96th, 120th hours interval to death. Results Positive of SPECT were found during 24th to 120th hour after the ^(131)I-C50 injection, Positive rates was 50%. The peak value of T/NT Tumor blood was 3.07±1.01 at 120th hour. But non-concentration of ()^(131)I-mIgG were found during 24th to 120th hour, which was distributed evenly in body. Conclusion C50 had affinity to breast carcinoma which over expressed CEA in nude mice bearing human breast Carcinoma. C50 is prospective to be used as the direct Carrier of breast carcinoma diagnosis.