摘要
分别构建了带有标记基因ＬａｃＺ和人的全长尿激酶原（ＰｒｏＵＫ）或纤溶酶原激活物（ｔＰＡ）基因的逆转录病毒载体，通过磷酸钙共沉淀法或假病毒感染法，转染血管平滑肌细胞，经Ｇ４ｌ８筛选得到抗性集落。分析证明，外源性基因可以整合到细胞染色体上，并可表达出相应的具有生物活性的蛋白质。提示血管平滑肌细胞可以作为心血管疾病基因治疗的靶细胞。
hree retroviral vectors containing report geneLacZ or the whole length cDNA of
human Pro-UKand tPA, pN2-LacZ, pN2-CMV-ProUK andpN2-CMV-tPA were constructed. The rat
vascularsmooth muscle cells (VSMCs) were transfected withthe plasmids by calcium phosphate
coprecipitation orpseudovirus infection. The transfected cells were se-lected by G418. Southern
blot analysis showed thatthe foreign genes were present in the genome of thetransfected VSMC,
and the expression products ofthese genes could be detected in the transfectedVSMCs. These
results suggested that the VSMC couldbe a targeting cell for gene therapy on
cardiovasculardiseases.
出处
《中华医学杂志》
CAS
CSCD
北大核心
1994年第4期224-227,共4页
National Medical Journal of China
基金
863资助
关键词
基因治疗
血管
平滑肌细胞
Gene therapy Gene expres-sion regulation Muscle, smooth,
vascular
