纳米马钱子碱脂质体制备工艺研究

Preparation of brusine liposomes

目的:采用薄膜超声分散法制备马钱子碱脂质体,考察了表面活性剂浓度,脂材比例,外水相条件,制备温度及药物浓度对马钱子碱脂质体粒径及包封率的影响。方法:薄膜超声分散法制备纳米级马钱子碱脂质体,凝胶色谱可见光比色方法测定包封率。结果:采用薄膜超声法制备的脂质体多为大单室脂质体,粒径主要分布在50～300nm。脂材比例为3∶10,表面活性剂Tween80与总脂材比为3∶10,制备温度为40°C,水相pH值为7.4时得到了粒径均匀、分散性好,包封率达61.24的马钱子碱脂质体。结论:薄膜超声法是制备纳米马钱子碱脂质体的一种简单可行的方法。

AIM: To study the influences of surfactant concentration, cholesterol concentration, the pH value of solution, preparation temperature, and drug concentration on the particle size and encapsulation efficiency of brusine nano-liposome. METHODS: The nano-liposomes was prepared by film-ultrasonic wave technology and the drug contents in liposomes were determined by gel chromatography-visible light chromatometry. RESULTS: By film-ultrasonic wave dissolving techniques, the large unilamellar liposome in the range from 50 nm to 300 nm was prepared. The optimum preparation technique was HPC and CHL in w/w ration of 3∶1, Tween-80 and total liposome in w/w ratio of 3∶10, at 40(°C) reactive temperature, 1 mg/mL drug concentration and solution pH7.4. The prepared brusine nano-liposomes demonstrated good decentralization and encapsulation efficiency high to 61.24%. CONCLUSION: Film-ultrasonic wave is a simple method to fabricate nano-brusine liposome.