微波预处理提取银杏叶黄酮的工艺研究和机理探讨

The process and mechanism of extracting flavonoids from Ginkgo Biloba Leaves by microwave pretreatment

目的:对微波预处理提取银杏黄酮的机理进行探讨。方法:以干银杏叶为原料,采用单因素实验分析微波预处理提取过程操作参数对提取结果的影响,借助电子显微镜观察细胞结构。结果:银杏叶经微波预处理后,不但细胞结构被破坏,而且有效成分得到充分解吸,后续提取速度比醇法快4倍,得率及浸膏纯度也比醇提法稍高。结论:微波预处理法具有提取速度快、有效成分得率高、微波利用率高等优点,值得推广。

AIM: The mechanism of extracting flavoinoids from Ginkgo biloba leaves by microwave prtreatment was studied systematically. METHODS: The dired leaves were used as the crude drug, the effect of the operation parameters during the extraction on the extraction result was analyzed with monofactor experiment, and spectrophotometer was used to observe the structure of the cells. RESULTS: After the microwave pretreatment, the structure of the leaves cell was destroyed and the effective composite was desorbed completely. The following extraction rate was 4 times higher that of traditional extraction, the yield and the purity were better than the ethanol extraction. CONCLUSION: The short extraction time, higher yield of effective composite, higher usage rate of the microwave, the method is worthy to be used. W