大孔树脂富集坤怡宁颗粒中芍药苷的工艺研究

Enrichment process of paeoniflorin from Kunyining Granules with macroreticular resin.

目的:研究大孔树脂富集坤怡宁颗粒(赤芍等)中芍药苷的工艺条件及参数。方法:以芍药苷为指标,考察大孔树脂富集坤怡宁颗粒中芍药苷的最佳工艺条件。结果:提取液上大孔树脂柱,吸附30min后,以蒸馏水洗去杂质,再以6倍上样量的50乙醇洗脱芍药苷为最佳工艺。结论:此法可较好的富集坤怡宁颗粒中主要有效成分芍药苷。

AIM: To study the optimal condition and parameters for the enrichment process of paeoniflorin in Kunyining Granules (Radix Paeoniae Rubra, etc.) with macroreticular resin. METHODS: With the enrichment degree of paeoniflorin in Kunyining Granules as an index, the optimal conditions of the enrichment process were investigated. RESULTS: The optimal condition showed that the aqueous extract was taken into the macroporous resin column, and kept for 30 min, then the resin was washed by water to get rid of impurity and then we used six times as much 50% ethanol as aqueous extract to elute paeoniflorin. CONCLUSION: The process is feasible to enrich paeoniflorin from Kunyining Granules.