丁型肝炎病毒中国SZ株的cDNA克隆及基因变异分析

Chinese strain of hepatitis D virus:molecular cloningand sequencing , and the significance of its genome di-vergence

以四川地区１例无症状携带丁型肝炎病毒（ＨＤＶ）和乙型肝炎病毒（ＨＢＶ）患者的外周血为标本，采用逆转录－ＰＣＲ技术，获得了ＨＤＶ全基因组的ｃＤＮＡ克隆（ＨＤＶＳＺ９３株），全长１６８４ｂｐ。与世界其他七个地区株相比，其核酸同源性为８１．８％～９５．４％；丁肝病毒抗原蛋白编码区核酸同源性为８８．９％～９６．１％，氨基酸同源性为８６，４％～９３．０％。另从１例慢性重型肝炎患者血清内克隆了丁肝病毒抗原蛋白（ＨＤＡｇ）编码基因（ＨＤＶ－ＳＺ９２株），与ＳＺ９３株相比，亦存在若干变异。对这些不同地区、不同病情ＨＤＶ株变异的可能意义进行了讨论。

he hepatitis D virus (HDV) obtained from achronic asymptomatic HDV/HBsAg carrier (SZ 93) inSichuan province was cloned with 1 684 nucleotides infull-length genome by reverse transcription-PCR.Comparison of SZ 93 with those obtained from differ-ent geographic area: i. e., Italia,United States,British, Nauru,Taiwan,Netherland, showed 81.8 %￣ 95.4% homology in nucleoude scquence of wholegenome, 88.9%￣96. 1% homology in nucleotide se-quence of HDAg-coding region,and 86. 4%￣93.0%homology in amino acid sequence of HDAg protein.Also, another isolate (SZ 92 ) obtained from a chronicsevere hepatitis patient in Sichuan was cloned withHDAg-coding region. There were some mutations inSZ 92compared withSZ 93as well as the other clonesmentioned above. The meaning for the genome diver-gence of HDV SZ 93 and SZ 92 was discussed.