P53基因突变与泌尿系肿瘤关系的初步研究

Mechanism of p53 gene mutation in development ofurologic neoplasms

采用ＤＮＡ聚合酶链式反应－单股构型多态分析及ＤＮＡ直接测序法对２０例膀胱移行上皮癌、１０例肾细胞癌和１７例睾九精原细胞瘤组织的Ｐ５３基因突变进行研究，有７例（３５％）膀胱癌和４例（２３．５％）睾丸精原细胞瘤有Ｐ５３基因突变，另外研究了抗癌基因Ｒｂ失活和癌基因ｒａｓ和ｃ－ｅｒｂＢ－２激活。结果提示泌尿系肿瘤的发生和Ｐ５３基因突变有关，也涉及Ｒｂ和ｒａｓ、ｃ－ｅｒｂＢ－２的异常改变。

ith DNA polymerase chain reactionsinglestrand conformation polyniorphism assay followed bydirect DNA sequencing. po3 gene niutation was ex-arnined in bladder transitional epithelial cell carcino-nia, renal cell carcinoma and testicular seminoma.po3 gene rnutation was found in 7 cases (35%) ofbladder carcinoma and 4 cases (23. 5%) of testicularseminoma. Inactivation of Rb gene and activation ofras and c-erbB-2 were also studied. The results sug-gest that development of urologic neoplasms is close-ly associated with P53 gene mutation and involvesloss of expression of Rb and aberrant expression ofras and c-erbB-2.