重组人肿瘤坏死因子诱导HL－60白血病细胞分化及调控原癌基因表达的作用

Low-dose recombinant human tumor necrosis-a inHL-60 leukemic cell line : effect on induced differen-tiation and regulat Expression of c-myc , c-fos proto-oncogenes

采用２０～２００Ｕ／ｍｌ的重组人肿瘤坏死因子（ｒｈＴＮＦ－α）处理体外液相培养的人早幼粒白血病细胞珠ＨＬ－６０，观察到５０～２００Ｕ／ｍｌ的小剂量ＴＮＦ具有诱导其向单核－巨噬细胞途径分化及抑制其增殖的效应。采用细胞总ＲＮＡ打点杂交技术检测１～１００Ｕ／ｍｌＴＮＦ诱导ＨＬ－６０细胞早期（１２小时内）对其ｃ－ｍｙｃ，ｃ－ｆｏｓ原癌基因表达的影响，发现ＴＮＦ可抑制ｃ－ｍｙｃｍＲＮＡ水平及ｃ－ｆｏｓ短暂性表达增高。结果表明此小剂量ｒｈＴＮＦ－α具有诱导白血病细胞分化的效应及调控多癌基因表达的作用。

he promyelocytic leukemic cell line HL-60 with20～200U/ml of low-dose rhTNF-a cultured in liquidculture system in vitro was used to observe the effecton HL-60 by TNF. TNF within dose of 50～200Ulml can induce HL-60 cell differentiation along themonocyticmarcrophage pathway , and inhibit HL～60cell prolifiretion. The total RNA of HL-60 cell wasused to hybrize to v-myc or vfos probe by dot blot.We detected the expression changes of c-myc or c-fosprotooncogene by 1～100U/ml of TNF inducingHI.60 cell for 1～12 hours. TNF could regulate thelevel of cmyc or c-fos mRNA, the transcription oftniyc was inhibrted remarkdly , and the expression ofcfos was increased early. The results indicated thatTNF in low-dose have effect on inducing HL-60 celldifferetiation and regulating expression of multionco-gene .