体外循环犬红细胞的脂质过氧化损伤及维生素E的保护作用

Lipid peroxidation injury to red blood cells duringextracorporeal circulation: mechanism and protec-tion

通过犬体外循环模型，以血浆和红细胞膜过氧化脂质（Ｐ－ＬＰＯ和Ｅｍ－ＬＰＯ）作为机体及红细胞膜过氧化损伤的指标，同时测定红细胞变形能力（ＩＦ）、血浆游离血红蛋白（ＰＦ－Ｈｂ）、红细胞超氧化物歧化酶（Ｅ－ＳＯＤ）和红细胞钠钾－ＡＴＰ酶及钙镁－ＡＴＰ酶。观察到体外循环对红细胞具有明显的损伤作用，ＩＦ及ＰＦ－Ｈｂ升高，分别与Ｐ－ＬＰＯ和Ｅｍ－ＬＰＯ呈直线正相关。维生素Ｅ可明显减轻脂质过氧化反应，对红细胞具有确切的保护作用。文章简要讨论了氧自由基对红细胞损伤的机制及维生素Ｅ保护作用的途径。

welve dogs were subjected to cardiopulmonarybypass with membrane oxygenator for 120 niinutes.The effect of lipid peroxide inJury on red blood cellswas studied by measurement of plasma and erythro-cyte menibrane lipid peroxide, deformabioity of ery-throcyte, plasma free hemoglobin , superoxide dismu-tase, Na￣+-K￣+-ATPase and Ca2+-Mg2+-ATPase oferythrocytes. The effect of vitamin E on red bloodcells was also investigated. The findings indicatedthat vitamin E might protect red blood cells fromlipid peroxide inJury during extracorporeal circulation. The mechanism of damage effect of lipid perox-ide and the protective effect of vitarnin E on red bloodcells were briefiy discussed.