氟虫腈急性中毒小鼠毒性和病理形态学改变

Experimental study on toxicity and pathological morphology in mice subjected to fipronil poisoning

目的:探讨氟虫腈急性中毒小鼠的毒性及病理学的改变。方法:用灌胃法,观察29.4mg/kg、36.8mg/kg、46.0mg/kg、57.6mg/kg、72.0mg/kg、90.0mg/kg等6个剂量组小鼠中毒后表现,计算氟虫腈经口半数致死量(LD50),通过光镜和电镜观察其脑、肺、胃、肝、心等重要脏器的病理形态学改变。结果:各剂量组均有小鼠出现抽搐、呼吸困难等临床症状;氟虫腈的经口LD50为54.98mg/kg,大体可见胃扩张,光镜下脑轻微水肿,电镜下可见神经元细胞核膜间隙轻度扩张、神经胶质细胞空泡化、神经纤维呈脱髓鞘样改变。结论:氟虫腈属中等毒性,急性小鼠中毒主要损害中枢神经系统,还可导致胃扩张。

Objective: To explore and discuss the toxicity and pathological changes in mice subjected to acute fipronil poisoning. Methods: Fipronil solution was given via gastric canal to different groups of mice at different concentration (29.4 mg/kg,36.8 mg/kg,46.0 mg/kg,57.6 mg/kg,72.0 mg/kg, and 90.0 mg/kg). The behavior of mice was observed. LD50 of fipronil was calculated. Pathological changes of lung,brain,stomach,kidney,sliver and heart were observed under light microscope and electon microscope. Results: The clinical symptoms,such as convulsion and dyspnea were seen in each group at different concentration. LD50 per os was 54.98 mg/kg. Gastrectasia,on the whole,could be found. Mild brain edema could be seen under light microscope. Slight dilation of the karyothecal gap in hippocampal neuron,vacuoles in the hippocampal neuroglia and demyelination in the nerve fibers could be seen under electron microscope. Conclusion: Acute poisoning of fipronil with moderate toxicity mainly damages the central nervous system,and can result in gastrectasia.