纳络酮对脑缺血再灌注海马细胞凋亡的影响

Nalxone decreases the ratio of apoptosis of the rabbit’s hippocampus cells induced by ischemia and reperfusion

目的:观察缺血再灌注期间海马细胞的凋亡及纳络酮对神经细胞的保护作用。方法:20只新西兰大白兔随机分成4组(n=5):正常对照组、单纯缺血组(缺血组,夹闭颈总动脉和椎动脉30min)、缺血再灌注组(再灌注组,夹闭颈总动脉和椎动脉30min后开放6h)、缺血再灌注加纳络酮干预组(纳络酮组,0.8mg/kg静注及0.3mg/kg静滴)。然后用流式细胞仪检测干预前、后的海马细胞的凋亡状况。结果:缺血组(17.87%±3.04%)、再灌注组(38.84%±12.86%)的细胞凋亡比率显著高于对照组(5.94%±0.59%,P<0.01);再灌注组又明显高于缺血组(38.84%±12.86%vs17.87%±3.04%)和纳络酮组(38.84%±12.86%vs8.96%±0.97%,P<0.01);而对照组与纳络酮组间则无显著差异。结论:①兔全脑缺血后30min出现海马细胞凋亡;再灌注6h后,上述改变加重。②纳络酮对缺血再灌注引起的神经细胞凋亡有一定的保护作用。

Objective:To study the protective effect of Nalonxe on the hippocampus cells of rabbits induced by ischemia and reperfusion. Methods:20 New Zealand white rabbits were randomly divided in four groups: control group, ischemia (bilateral common carotid artery and vertebral artery were occluded for 30mins) group, ischemia-reperfusion(above mentioned occluded artery were opened for 6hours) group, reperfusion associated with Naloxone therapy(0.8mg/kg iv and then 0.3mg/kg vd.) group. At the end of intervention, the ratio of apoptosis of hippocampus cells were assayed by flow cytometry. Results:1.The ratio of apoptosis of ischemia group and ischemia-reperfusion group were all significant higher than control group(P<0.01 respectively); and the ratio of ischemia-reperfusion group was significant higher than ischemia and naloxone group(P<0.01 respectively). (2. There) was no remarkable difference between that of naloxone therapy group and control group.Conclusion:Ischemia and ischemia-reperfusion may lead to apoptosis of hippocampus cells. Naloxone significantly reduces apoptosis of hippocampus cells and provide protective effect on hypoxia and reperfusion injure.