帕金森模型大鼠海马区神经干细胞增殖情况的实验研究

The proliferation of neural stem cells in hemi-parkinsonian rats

目的:制作帕金森大鼠模型,研究其海马区神经干细胞的增殖情况。方法:6羟多巴胺纹状体内注射制作大鼠帕金森模型,于不同时间点处死,处死前均注射5溴脱氧尿苷(Brdu)。免疫组织化学方法动态检测Brdu和巢蛋白(Nestin)的表达。Brdu标记方法确定脑内海马区增殖的细胞,Nestin的表达用于确定脑内海马区神经前体细胞,Brdu/Nestin免疫双标确定脑内海马区神经干细胞的增殖情况。结果:同正常组对照组,假手术对照组相比较,PD大鼠海马区的Brdu+细胞,Nestin+细胞和Brdu+/Nestin+细胞数在模型成功后的第3、5、7天明显增加,第14天后逐渐减少,第28d后恢复正常水平。且损伤对侧的Brdu+/Nestin+细胞数也明显增加,但少于损伤侧。结论:6OHDA纹状体内注射制作的PD模型大鼠能够使大鼠海马区的神经干细胞增殖。

Objective:To investigate the proliferation of neural stem cells in the rat model of Parkinsons disease .Methods:Rat model of Parkinsons disease was made by injecting the 6-hydroxydopaminin in the striatum.The rats were killed at different time points,and their brain were taken out.Before they were killed ,the rats were injected Brdu into their abdomen.The dynamic expression of Nestin and Brdu were determined by immunocytochemistry,Brdu labeling method was used to mark the cells of proliferation .Nestin expression was used to identify neuroprogenitor cells.And they both used to mark the diving neural stem cells.Rsults:Campared with the controls,the number of Nestin-positive cells ,Brdu-positive cells,and Brdu-Nestin-positive cells increased strikingly at 3,5,7days,in the hippocampus, then declined at 14 days and almost reached the normal 28 days after.(Conclusion:)The rat model of Parkinsons disease by direct injection of 6-OHDA in the striatum stimulates the proliferation of inherent neural stem cells.