乳内动脉桡动脉大隐静脉的内皮型一氧化氮合酶表达

Expression of endothelial nitric oxide synthase in the human internal mammary arteries, radial arteries and saphenous veins used in coronary artery bypass grafting

目的 应用免疫组织化学方法,探讨内皮型一氧化氮合酶(eNOS)在冠状动脉旁路移植术中常用的3种血管材料乳内动脉(LIMA)、桡动脉(RA)及大隐静脉(SV)中的定位及表达。方法 取2 0例行冠状动脉旁移植术(CABG)的冠心病病人的LIMA、RA及SV的废弃血管标本,行光镜观察及eNOS的免疫组织化学染色进行半定量观察。并行计算机图像分析,比较灰度值。结果 eNOS在3种血管材料的内膜均有明显表达,LIMA灰度值明显高于RA及SV ,RA灰度值高于SV ;LIMA、RA的肌性中层可见eNOS的表达而SV无表达,LIMA灰度值明显高于RA。结论 各种血管材料中eNOS的不同表达。

Objective To investigate the localization and expression of endothelial nitric oxide synthase (eNOS) within the wall of the human internal mammary arteries (LMA), radial arteries (RA) and the great saphenous veins (SV) using immunohistochemistry method. Methods Specimens were harvested from 20 patients undergoing coronary artery bypass grafting and submitted to light microscope analysis using immunohistochemistry method. Results The expression of eNOS was evident in the intima of LIMA, RA and SV and in the media of muscular LIMA and RA. No eNOS expression was found in the media of great saphenous veins. Semiquantitative and the imaging analysis indicated by gray level values revealed a higher eNOS expression in the wall of internal mammary artery, particularly at the level of the media. Conclusion Different expression of eNOS in the intima and media of LIMA, RA and SV may provide a histological explanation for the better results of the LIMA when used for coronary artery bypass grafting.