抑肽酶对缺血肺组织ICAM-1和P-选择素基因mRNA表达的影响

The influence of aprotinin on the mRNA expression of P-selectin and ICAM-1 in the in situ lung tissue after ischemic cold storage and reperfusion

目的 探讨抑肽酶对在体缺血冷存再灌注后肺组织细胞间黏附分子 1(ICAM 1)和P 选择素基因mRNA表达的影响。方法 新西兰白兔30只,随机分为3组:对照组、PLD组和抑肽酶组。建立兔在体肺缺血冷存再灌注模型,对照组左肺不灌注肺保护液,阻断左肺门后直接将左肺下叶在体冷存于10℃肺保存器内2h ,再灌注2h ;LPD组左肺门阻断后经肺动脉灌注LPD液,余同对照组;抑肽酶组灌注液为含抑肽酶的改良LPD液,余同LPD组。分别于左肺门阻断前、缺血2h、再灌注2h取左肺组织,以RT PCR技术检测P 选择素和ICAM-1基因mRNA表达。结果 再灌注2h ,对照组和LPD组P 选择素基因mRNA的表达量明显高于缺血前和缺血2h ,抑肽酶组则无明显变化。缺血2h和再灌注2h ,对照组和LPD组ICAM-1基因mRNA表达量较缺血前均显著升高,且明显高于抑肽酶组。结论 抑肽酶可抑制缺血冷存再灌注后肺组织P 选择素和ICAM-1基因mRNA表达上调。

Objective To explore the influence of aprotinin on the mRNA expression of P-selectin and ICAM-1 in lung tissue after ischemic reperfusion. Methods Thirty New Zealand white rabbits were randomly divided into 3 groups: control group, LPD group and aprotinin group. In situ rabbit lung preservation model was established. In control group, the left lower lung lobe was stored at 10℃ in a specially made lung preservation container for 2 hours and reperfused for another 2 hours. In LPD group and aprotinin group, the left lower lobe was perfused with LPD solution or aprotinin containing LPD solution, respectively, after left lung hilus was clamped. The other procedures were the same as control group. The lung tissue was harvested at different time intervals including pre-clamping lung hilus, 2 hours after clamping and 2 hours after reperfusion. The mRNA expression of ICAM-1 and P-selectin in the lung tissue was detected with RT-PCR technique. Results The contents of mRNA of P-selectin at 2 hours after reperfusion in control group and LPD group were significant higher than pre-ischemia and 2 hours after clamping the left lung hilus. There was no such significant difference in aprotinin group. The mRNA expression of P-selectin in aprotinin group at 2 hours after reperfusion was significantly lower than that in control group and LPD group. The ICAM-1 mRNA expression at 2 hours after Ischemia and 2 hours after reperfusion in control group and LPD group was significantly higher than that pre-ischemia and it was significantly higher than that in aprotinin group. Conclusion Aprotinin can inhibit the upregulation of the mRNA expression of P selectin and ICAM-1 after ischemia reperfusion in the lung tissue, so the addition of aprotinin in LPD solution may reduce the Ischemia reperfusion injury in lung tissue.