粉防己碱对缺血心脏去甲肾上腺素释放及代谢的影响

Effect of tetrandrine on cardiac noradrenaline release and metabolize induced by electrical field stimulation

目的探讨不同浓度粉防己碱对离体豚鼠心脏缺血时去甲肾上腺素(NE)释放及其在轴浆内代谢的影响。方法用高效液相色谱-电化学法测定豚鼠离体心脏灌注流出液中NE和二羟基苯基乙二醇(DHPG)的含量,将正常灌注下(S1)和不同条件缺血灌注下(S2)两刺激进行自身前后对比。结果各浓度组S2刺激期间NE的释放量较缺血对照组明显减少(P<0.05),且各浓度组间均有显著性差异(P<0.05);DHPG的量在缺血对照组和低浓度组间并无显著性差异(P>0.05),而中浓度和高浓度组DHPG的量较对照组和低浓度组有了明显升高(P<0.05),且高浓度组与中浓度组间有显著性差异(P<0.05)。结论粉防己碱可以明显抑制缺血时离体豚鼠心脏NE的释放,其作用随浓度升高而增强,而较高浓度的粉防己碱在抑制NE释放的同时还增强了轴浆中NE的代谢,且此作用也随浓度升高而增强。

Objective To investigate the effect of Tetrandrine(Tet) in different concentrations on Cardiac Noradrenaline(NE) Release and Metabolize Induced by Electrical Field Stimulation in isolated perfused gunia pig hearts under ischemic conditions.Methods Method High-performance liquid chromatography(HPLC) with electrochemical detection was used to determine the outflow of cardiac NE and dihydroxyphenylglycol(DHPG) from perfused guinea pig hearts. Electrical field stimulations were applied twice(S_1 ,S_2 ) under different conditions.Results Tet revealed a concentration dependent decrease of NE release at concentrations of 10 μmol/L , 30 μmol/L , 80 μmol/L under ischemic conditions ( P < 0.05 ).There was no statistical difference in DHPG release between ischemic,whereas the release of DHPG was markly increased in middle and high concentration group( P < 0.05 ).Conclusion Tet was able to inhibit the cardiac exocytotic NE release in isolated perfused gunia pig hearts under ischemic conditions.In middle and high concentration,it can even accelerate the metabolizing of the axoplasmic NE. Those effects of Tet were related with its concentration.