2型糖尿病大鼠心肌病变模型的制备(英文)

Preparation of cardiomyopathy model in rats with type 2 diabetes

背景:糖尿病心肌病变的结局与糖尿病患者的高心力衰竭发生率密切相关,建立良好的糖尿病心肌病变动物模型为其改善心功能研究极有意义。目的:通过建立2型糖尿病的大鼠及糖尿病心肌病变模型,了解高血糖状况对心肌的影响。设计:以实验动物为研究对象,完全随机分组设计,随机对照研究。单位:一所大学医院内分泌科。方法:实验选用新生雄性Wistar大鼠99只,随机将其分为实验组75只和对照组24只。对照组腹腔注射柠檬酸钠缓冲液,实验组一次性腹腔注射链脲佐菌素90mg/kg,10周后检测糖耐量以确定糖尿病模型的建立;处死后电镜检查其心肌的超微结构。主要观察指标:观察糖尿病大鼠心肌细胞肌原纤维以及线粒体是否改变。结果:实验组空腹血糖犤(8.87±0.76)mmol/L犦轻度高于对照组犤(5.88±0.45)mmol/L犦;实验组餐后2h血糖犤(12.71±0.53)mmol/L犦明显高于对照组犤(6.42±0.4)mmol/L犦,糖耐量异常;电镜下出现肌原纤维呈局灶性变性、肌丝排列紊乱,心肌细胞线粒体肿胀、局灶性增生等改变。结论:该模型是研究2型糖尿病及其心肌病的一种理想载体,为研究其心功能奠定了实验学基础。

BACKGROUND:Diabetic cardiomyopathy is closely related to the high incident rate of diabetic heart failure.It is extremely significant to set up the successful dietetic cardiomyopathy model for the study on improving cardiac function. OBJECTIVE:To understand the influence of high blood sugar on myocardium by setting up the model of type 2 diabetes and diabetic cardiomyopathy.DESIGN:Completely randomized design, randomized and controlled study,in which, the experimental animals were taken as the objects.SETTING:Department of endocrinology of a university.METHODS:In the experiment, 99 newly born Wistar male rats were employed,randomized into experiment group (75 rats) and the control group(24 rats).In the control group,abdominal injection was done with sodium citrate buffer and the single abdominal injection was done with strptozocin(STZ) 90 mg/kg in the experiment group. Ten weeks later, the glucose tolerance was assayed to determine the set up of diabetes model.The ultrastructure of myocardium of rats was tested under electronic microscope after being sacrificed. MAIN OUTCOME MEASURES:To observer whether the myofibrillae and mitochondria of myocardiocytes alter or not in diabetic rats.RESULTS:In the experiment group, the fasting blood sugar[(8.87 ± 0.76) mmol/L] was slightly higher than that[(5.88± 0.45) mmol/L] in the control group, the blood sugar 2 hours after meal was remarkably higher than that in the control group and the glucose tolerance was abnormal.Under the electronic microscope,the myofibrillae presented focal degeneration and disarranged order of myofilament;mitochondria of myocardiocytes presented swelling and focal proliferation.CONCLUSION:This model is the satisfactory carrier for the study on diabetes II and its myocardiopathy,which lays an experimental foundation for the study on cardiac function of type 2 diabetes.