纳米磁化标记神经干细胞的MRI大鼠活体示踪实验研究

In vivo Magnetic Resonance Imaging Tracking of Transplanted Nanometer SPIO-labeled Neural Stem Cells in Rats

目的:探讨用MRI活体示踪移植磁化标记神经干细胞在创伤性脑损伤模型大鼠脑内迁移和分布的可行性。方法:建立大鼠脑部左侧半球脑损伤模型。2周后将磁化标记胚胎神经干细胞立体定向移植入大鼠脑部右侧半球。在移植后1、3d及1、2周分别行大鼠头部MRI。结果:移植后行头颅MRI可见移植部位FSET2 WI和GRET2 序列呈环形低信号。实验组大鼠头颅MRI脑内有一低信号线,指向对侧脑挫伤部位。结论:用MRI活体示踪移植磁化标记神经干细胞在TBI模型大鼠脑内迁移和分布是可行的。

S Aim:To study the feasibility of magnetic resonance imaging tracking of transplanted nanometer SPIO-labeled neural stem cells in vivo in rats with traumatic brain injury (TBI) model. Methods: TBI model was built in the left hemisphere of the rats. In vitro embryonic neural stem cells were labeled by nanometer SPIO. 2 weeks later,SPIO-labeled embryonic neural stem cells were implanted into the right hemisphere .All rats were performed MRI 1 day,3 days,1 week and 2 weeks after implantation. Results: At implanted sites low signal intensity could be observed on MRI examination with the scanning sequences of FSE T_2WI and GRE T_2 .Low signal intensity lines could be observed targeting to the contralateral non-injuried areas in experimental group.Conclusion: MRI in vivo monitoring transplanted nanometer SPIO-labeled neural stem cells is effective in rats with TBI model.