摘要
目的:研究原代培养的大鼠胚胎中脑前体细胞(mesencephalicprogenitorcells ,MPC)作为帕金森病(Parkinson’sdisease ,PD)细胞替代疗法供体的可行性。方法:PD模型大鼠19只,随机分为细胞移植组(n =9)、假移植组(n =5 )和对照组(n =5 ) ;定期测定移植前后各模型鼠阿扑吗啡诱导的旋转行为的变化,免疫组化定性供体细胞在宿主体内存活和分化的情况。结果:术后6和16周,细胞移植组阿扑吗啡诱导旋转的相对频率与术前比较有显著差异;细胞移植组术后16周时的阿扑吗啡诱导旋转的相对频率平均值与对照组对应的相对频率平均值相比也有显著差异;供体细胞能在宿主体内分化为多突起的DA能神经元。结论:原代培养的胚胎MPC可能作为PD患者细胞替代治疗的供体细胞。
Aim:To study the feasibility of in vitro expanded mesencephalic progenitor cells(MPC) of rats being used as a new approach in cell replacement therapy of Parkinson's disease.Methods:In vitro expanded MPC of E12 rats was trypsinized after 5 to 7 days in vitro and re-suspended in D-Hank's solution at a density of 120,000 viable cells per microlitre.In cell grafted group ( n =9),four microlitre cell suspension was grafted into the lesioned stritum of hemiparkinsonian rat;in sham transplanted group ( n =5),four microlitre D-Hank's solution was injected in the same way;the other 5 hemiparkinsonian rats served as a control group.The apomorphine-induced rotation test were recorded regularly,the survival and differentiation of grafted cells were evaluated by immunohistochemistry at 6 months post-transplantation.Results:The cell grafted rats exhibited a marked reduction in relative apomorphine-induced rotation rate at 6 and 16 weeks post-transplantation.Compared with the control group,the average relative apomorphine-induced rotation rate in cell grafted group at 16 weeks post-transplantation was decreased significantly too.The grafted cells could differentiated into large,multipolar dopamine(DA) neurons in lesioned stritum and survived more than 6 months after intrastritum transplantation.Conclusion:In vitro expanded MPC of E12 rats could differentiated into DA neurons in the lesioned stritum and had a pronounced effect on apomorphine-induced turning behavior to the hemiparkinsonian rat.It might served as a new donor in cell replacement therapy of Parkinson's disease.
出处
《中国临床神经科学》
2005年第2期193-197,共5页
Chinese Journal of Clinical Neurosciences
