SKP2、P27^(KIP1)蛋白表达与胃癌的临床关系

Expression and Clinical Significance of SKP2 and P27^(KIP1) Proteins in Human Gastric Cacinoma

目的探讨细胞S相激酶相关蛋白(SKP2)、P27KIP1蛋白表达与胃癌的临床关系。方法用免疫组化SP法检测69例胃癌中SKP2、P27KIP1蛋白的表达。结果69例胃癌组织中P27KIP1、SKP2蛋白表达率分别为46.38%、33.33%;随肿瘤分化程度降低、浸润深度加深、恶性程度增加,P27KIP1阳性表达率逐渐降低(P<0.05),SKP2阳性表达率随肿瘤分化程度降低而升高;二者的阳性表达率均与胃癌患者的年龄、性别、肿瘤大小及部位无关(P>0.05)。P27KIP1与SKP2表达呈显著负相关(P<0.01)。结论P27KIP1、SKP2异常表达可加速细胞周期转化,促进胃癌的发生,其蛋白检测尤其是P27KIP1蛋白检测有助于胃癌恶性程度和预后的判断;泛素-蛋白酶体途径可能在胃癌发生中扮演着重要角色。

Objective To investigate the clinical significance of P27~ KIP1 and S-phase kinase-associated protein 2 (SKP2) expression in human gastric carcinoma. Methods The expression of P27~ KIP1 and SKP2 was determined by SP immunohistochemical method in 69 specimens of gastric carcinoma. Results The positive rates of P27~ KIP1 and SKP2 expression were 46.38% and 33.33%, respectively. The positive rate of P27~ KIP1 expression in gastric carcinoma decreased with the poor differentiation,deep invasion and progression of pathological grade (P<0.05),while the positive rate of SKP2 expression increased when the differential degree of gastric carcinoma decreased. The positive rate of both the P27~ KIP1 and SKP2 expression was not associated with the patients age, sex,and tumor size and location (P>0.05). There was a negative correlation between the P27~ KIP1 and SKP2 expression(P<0.01). Conclusion The abnormal expression of P27~ KIP1 and SKP2 may promote cell cycle progression and gastric carcinogenesis. Detecting P27~ KIP1 and SKP2 proteins expression, especially P27~ KIP1, may be helpful for evaluating the malignant degree and prognosis of gastric carcinoma. Ubiquitin-proteasome pathway may play an important role in gastric carcinogenesis.