运用流式细胞术对乳腺癌激素受体检测的研究

Detection of Estrogen Receptor in Breast Cancer by Flow Cytometry

目的运用双参数流式细胞术检测临床乳腺癌新鲜标本单细胞悬液的雌孕激素受体的状态,同时结合DNA分析倍体和S期的比例判断乳腺癌患者的预后。方法获取手术切除的临床乳腺癌新鲜标本32例,制成单细胞悬液后,运用双参数流式细胞术检测雌孕激素受体的状态,并分析倍体情况和S期比例。结果⑴运用流式细胞术不仅能定性而且能定量检测临床标本的雌孕激素受体状态,且所需组织量少,这种方法比免疫组化的方法更敏感。⑵同时结合DNA,判断乳癌的倍体和S期的比例。结论流式细胞术检测激素受体更敏感、更具特异性,能更确切地指导临床医生的判断和治疗。

Objective To detect estrogen receptor and DNA index in breast cancer by flow cytometry. Methods 32 cases of fresh specimens of breast cancer resected by operation were used in this study. Single cells suspension was prepared from those specimens, and estrogen receptor expression was analyzed by flow cytometry. At the same time, the status of ploid and S phase cell ratio were determined. Results Flow cytometry analysis could measure the expression level of estrogen receptor in the fresh breast cancer specimens, which was more sensitive and needed less volume of tumor tissue compared with immuohistochemical method. The information on the status of ploid and S phase cell ratio were simultaneously obtained. Conclusion The detection of estrogen receptor in breast cancers by flow cytometry was more helpful for evaluating prognosis and selecting treatment.