高效液相色谱法测定鱼腥草中金丝桃苷与槲皮苷的含量

Quantitative Determination of Hyperoside and Quercitrin in Houttuynia cordata by HPLC

目的用高效液相色谱法测定鱼腥草中金丝桃苷与槲皮苷的含量。方法采用ShimadzuC18柱(150mm×4.6mm,5μm),流动相为甲醇-0.2%H3PO4水溶液(45:55),流速1.0mL.min-1,检测波长350nm,柱温室温,用外标法定量,测定鱼腥草中金丝桃苷与槲皮苷的含量。结果金丝桃苷的线性范围0.50-2.50μg,r=0.9998,回收率102.47%,RSD1.04%;槲皮苷的线性范围0.40-2.00μg,r=0.9996,回收率97.92%,RSD2.13%。结论该方法简便,快速,线性关系良好。

Objective The purpose is to determine the content of hyperoside and quercitrin in Hout-tuynia cordata. Method The HPLC method was used to determine the content of hyperoside and quercitrin in Houttuynia cordata. The analysis was carried out on Shimadzu C18 column(150 mm×4. 6 mm,5 μm). The mobile phase was MeOH-0. 2%H3PO4 (45:55). Flow-rate was1. 0 mL. min-1. Wave-length was 350 nm. Temperature was room temperature. Result The method was simple and had a good linear relationship. The liner range of hyperoside is 0. 50-2. 50 μg and gave a correlation (r) of 0. 999 8. The recovery was 102. 47%. RSD was 1. 04%. The liner range of quercitrin is 0. 40-2. 00 μg and gave a correlation (r) of 0. 999 6. The recovery was 97. 92%. RSD was 2. 13%. Conclusion The method was easy to determine the content of hyperoside and quercitrin in Houttuynia cordata.