全胚胎体外培养方法筛查致畸原的应用

Whole Embryo Culture for Detecting Teratogen

全胚胎体外培养技术是将孕第９．５天的大鼠胚胎从子宫移至体外培养，培养期间在培养基中加入不同的化合物，由于排除了母体和胎盘因素，可以观察到这些化合物对胚胎生长发育的直接影响。应用该技术研究了青霉素、敌枯双、放线菌素Ｄ和高浓度葡萄糖四种化合物对胚胎生长发育的直接影响，结果发现在体外培养时也可导致胚胎死亡、畸形和胚胎生长发育迟缓，所导致的畸形与在整体动物实验结果相同。同时发现化合物在导致胚胎死亡、畸形和生长发育迟缓方面具有明显的剂量－效应关系。通过比较这种化合物导致胚胎死亡、畸形和发育迟缓的剂量－效应曲线的关系，推测其导致胚胎畸形的可能性以及畸形胚胎存活的可能性，从而推测化合物在实际中真正致畸的可能性。

hole embryo culture technique was used to study direct effects of Penicillin G,Thiadiazole,Actinomycin D and glucose solution of high concentration on the development and growth of rat em bryos,without maternal and placental influence.Nine-and-a-half-day rat embryos were transplanted to culture flasks from the animal′s wombs for in vitro culture,and the four chemicals were added to cul-ture medium,respectively.Results showed all those chemicals could cause death and malformation of the embryos and retardation in embryonic development and growth cultured in vitro,with an apparent dose-effect relationship. The embryonic malformations caused by the chemicals were the same both in vitro culture and in vivo.Possible teratogenesis of the chemicals and survival of the malformed embryos were speculated based on the above dose-effect relationship.