1,6-二磷酸果糖镁在毕格犬体内的药动学研究

Pharmacokinetics of fructose 1,6 diphosphate magnesium in Beagle dogs

目的测定毕格犬静脉注射不同剂量1,6-二磷酸果糖镁(FDP-Mg)后,FDP-Mg在毕格犬体内的药动学特点。方法12只毕格犬随机分成3组,分别静脉注射给予120,60,30mg/kgFDP-Mg,在设定的时间点采血,酶法及血清Mg2+测定试剂盒分别测定毕格犬血清中的FDP及Mg2+的浓度,DAS程序计算药动学参数。结果毕格犬静脉注射FDP-Mg后,Mg2+的血药浓度-时间曲线符合二室开放模型。其t1/2α为4.3-7.8min,t1/2β为90.5-180.8min,分布容积(Vd)为3.74-5.66L/kg,显示非剂量依赖性特征。FDP在血清中代谢迅速,末端半衰期t1/2el为97.4-128.7min,MRT为135.1-179.9min,CL为0.02-0.05L/(kg·min)。结论FDP和Mg2+的药动学特征差异明显,表明FDP-Mg在体内解离成FDP和Mg2+两部分后按照各自的代谢特征从机体内消除。

Purpose To study the pharmacokinetics of iv fructose 1,6 diphosphate magnesium in Beagle dogs. Methods 12 Beagle dogs were divided into 3 groups. A single dose of 120,60 and 30 mg/kg of fructose 1,6 diphosphage magnesium (FDP-Mg) was given by iv. Serial blood samples were collected at designed time points.The serum FDP concentration was measured by enzymology method and serum Mg2 + concentration was detected by serum Mg+determined kit. Pharmacokinetic parameters were computerized with DAS program. Results After intravenous of single dose FDP-Mg, the serum concentration versus time curves of Mg2+ conformed to two-compartment model. t1/2a was 4.3-7.8 min,t1/2βwas 90.5-180.8 min and Vd was 3.74 -5.66 L/kg.The main pharmacokinetic parameters of FDP were different from those of Mg2 + .Non-compartment model was used to calculate the main pharmacbkinetics parameters of FDP. t1/2el was 97.4-128.7 min, MRT was 135.1-179.9 min and CL was 0.02-0.05 L/(kg·min) . Conclusion The difference of the parameters between FDP and Mg2+ suggested that FDP-Mg could be divided into two parts,and then,they could be eliminated from the organism according to their own routes respectively.