地塞米松对哮喘豚鼠肺及内脏感觉传入系统神经生长因子表达的抑制作用

Inhibitory effect of dexamethasone on expression of NGF in lung and visceral sensory afferent system of asthmatic guinea pigs

目的:探讨地塞米松对哮喘豚鼠肺及内脏感觉传入系统(C7T5脊神经节及对应的脊髓后角)神经生长因子(NGF)表达的影响。方法:利用免疫组织化学和Western印迹方法,观察生理盐水组、单纯致敏组、哮喘组和地塞米松组豚鼠肺及内脏感觉传入系统(C7T5脊神经节及对应的脊髓后角)NGF表达的变化。结果:哮喘组豚鼠NGF在肺及内脏感觉传入系统(C7T5脊神经节及对应的脊髓后角)表达明显高于对照组,而地塞米松组则明显低于哮喘组。结论:NGF可能参与哮喘的发病过程,地塞米松抑制哮喘豚鼠NGF的表达可能是其治疗哮喘的机制之一。

Objective: To explore the effect of dexamethasone on the expression of NGF in the lung and the visceral sensory afferent system (C_ 7-T_ 5 spinal ganglia and the corresponding dorsal horn of the spinal cord) of asthmatic guinea pigs. Methods: The alterations of NGF expression were observed by means of immunohistochemistry and Western blot in the lung, C_ 7-T_ 5 spinal ganglia and the corresponding dorsal horn of the spinal cord of all guinea pigs. Results: The expression of NGF in the lung, C_ 7-T_ 5 spinal ganglia and the corresponding dorsal horn of the spinal cord of asthmatic guinea pigs was much higher than that of the control groups and the expression of NGF of the dexamethasone group was much lower than that of the asthmatic group. Conclusion: The present results indicate that the over-expression of NGF might be involved in the pathogenesis of the bronchial asthma, and inhibition of the expression of NGF by dexamethasone may be one of the mechanisms in the treatment of asthma.