那如-3巴布剂的抗炎镇痛作用研究

Anti-inflammatory and analgetic effects of Naru3 Cataplasm

目的观察那如-3巴布剂对大鼠佐剂性关节炎的抗炎作用和对小鼠的镇痛作用。方法建立大鼠佐剂性关节炎(AA)模型,用排水容积法测量大鼠足跖和关节的容积,计算其足跖肿胀度;用ELISA法测定大鼠血清白细胞介素1(IL1)、肿瘤坏死因子α(TNFα)和前列腺素E2(PGE2)水平;用醋酸致小鼠扭体法和热板法测痛阈。结果那如-3巴布剂对AA模型大鼠的急性炎症有明显的抑制作用,从第6小时起足跖容积和足跖肿胀度与模型组比较明显下降,对弗氏完全佐剂(FAC)所致的继发性炎症有明显抑制作用,与模型组比较,抗炎作用在第8天开始明显,第24天已非常明显,同时大鼠血清的IL1、TNFα和PGE2的水平下降。那如-3巴布剂还能明显提高小鼠的痛阈和疼痛抑制率。结论那如-3巴布剂对FAC所致的急性炎症和继发性炎症有明显的抑制作用,并有明显的镇痛作用。

Objective To observe the anti_inflammatory effect of Naru3 Cataplasm on adjuvant arthritis(AA) and its analgetic effect on mice.Methods To establish the rat model of AA,measure the volume of rat toe and joint and calculate the swelling degree of rat toe by the volume drainage method.The levels of interleukin_1(IL_1),tumor necrosis factor α(TNFα)and prostaglandin E_2(PG E_2) were determined by ELISA method,and the threshold of pain was detected by torsion method induced by acetic acid and hot_plate method.Results Naru3 Cataplasm inhibited obviously the acute inflammation in AA rats.The toe volume and swelling degree decreased at 6^(th) hour compared with that of the model group.Naru3 Cataplasm inhibited also the secondary inflammation induced by Freund's Adjuvant,Complete(FAC).Its anti_inflammatory effect began to be obvious at 8^(th) day and very obvious at 24^(th) day.At the same time the levels of IL_1,TNFα and PGE_2 in rat serum decreased.Naru3 Cataplasm increased remarkably the threshold of pain and pain_inhibition rate in mice.Conclusion Naru3 Cataplasm has an obvious inhibition effect on acute or secondary inflammatory induced by FAC and analgesia effect.