必需脂肪酸对大鼠脑组织中EPA、DHA含量的影响及意义

Effects and significance of essential fatty acids on contents of eicosapentaenoic acid and docosahexaenoic acid in rat brain tissues

目的 探讨在大鼠脑发育期补充必需脂肪酸(essentialfattyacid ,EFA)对脑组织中二十碳五烯酸(eicosapentae noicacid ,EPA)、二十二碳六烯酸(docosahexaenoicacid ,DHA)含量的影响。方法 断乳大鼠(1月) 3 0只,雌雄不拘,分为正常组、EFA缺乏组和补充鱼油组,分别饲以不同饲料喂养3个月,取出新鲜的脑组织,采用高效液相色谱法(highperformanceliquidchromatography ,HPLC)测定EPA、DHA含量的变化。结果 与正常组相比,EFA缺乏组的EPA、DHA含量均显著降低(P <0 0 5 ) ,补充鱼油组明显升高(P <0 0 5 )。结论 发育期EFA缺乏会导致脑组织中EPA、DHA含量明显降低,相反在发育期补充必要的EFA会使EPA、DHA明显升高,有利于脑组织的生长与发育。

Objective To investigate the effects of essential fatty acids(EFA)on the contents of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) in the brain tissues of rats. Methods A total of 30 neonatal rats, 1 month old, were randomly divided into normal, EFA deficiency and fish oil supplement groups and given different feeding stuff for 3 months respectively. The contents of EPA and DHA in brain tissues were determined by high performance liquid chromatography (HPLC). Results As compared with normal group, the contents of EPA and DHA in EFA deficient group were significantly decreased(P<0.05), but increased significantly in fish oil supplementation group. Conclusion Deficiency of EFA at developmental period can decrease the content of EPA and DHA. In reverse, supplement of EFA during the development can increase the EPA and DHA content and are beneficial to the growth and development of brain tissue.