荷负电气溶胶对大鼠烫伤创面表皮细胞生长因子和β_1整合素表达的影响

Influence of aerosol bioelectricity on expression of epidermal growth factor and β_1 integrin in healing wounds of burned rats

目的 研究荷负电气溶胶(aerosolbioelectricity ,ABE)对大鼠烫伤创面愈合过程中表皮细胞生长因子(epider malgrowthfactor ,EGF)和β1 整合素表达的影响,探讨荷负电气溶胶促进创面愈合的作用机制。方法 制作SD大鼠深Ⅱ度烫伤模型,将40只鼠随机分为治疗组(n1 =2 0 )和对照组(n2 =2 0 )。治疗组应用荷负电气溶胶治疗,每次1 5h ,每天2次,直至创面愈合;对照组不作荷负电气溶胶治疗。采用免疫组织化学和图像分析方法,检测创面愈合过程中EGF和β1 整合素表达水平。结果 创面平均愈合时间治疗组为(7±1 15 )d ,对照组为(9±1 3 4)d ,治疗组创面愈合时间明显提前(P <0 0 1)。免疫组化显示,治疗组伤后第2天可见EGF、β1 整合素开始表达于基底细胞和毛囊细胞,第5天表达显著,第6～9天持续高水平表达;对照组于第3天开始表达,第7天表达显著,第8～10天持续高水平表达。治疗组表达水平明显高于对照组(P <0 0 1)。创面愈合后两组表达逐渐恢复至正常水平,差异无显著性意义(P >0 0 5 )。结论 通过上调EGF和β1整合素的表达而促进细胞增殖可能是荷负电气溶胶促进烧伤创面愈合的机制之一。

Objective To investigate the influence of aerosol bioelectricity on the expressions of epidermal growth factor (EGF) and β_1 integrin in wound healing of burned rats. Methods Sprague Dawley (SD) rats were inflicted to TBSAⅡ burn by scalding. Rats were randomly divided into experimental group(n=20)and control group(n=20). The experimental group was treated with aerosol bioelectricity. Wound samples were collected in 1-10 d after scalding. Immunohistochemistry and image analysis methods were conducted to examine the expressions of EGF and β_1 integrin in both experimental and control groups. Results The average wound healing time of experimental group were (7±1.15) d, whereas (9±1.34) d in control group. The expression of EGF and β_1 integrin in experimental group were detected in skin basal cells and hair follicle cells 2 d after scalding, increased evidently in 5 d and sustained at high expression level up to 9 d after treatment. In contrast, the expressions of EGF and β_1 integrin were initially observed in control group in day 3 and sustained a rather high level in day 7 after scalding. The expressions of EGF and β_1 integrin was significantly higher in experimental group than in control group(P<0.01). Conclusion Aerosol bioelectricity can indicate active cells proliferation through up-regulating the expressions of EGF and β_1 integrin in skin basal cells and hair follicle cells, thus can accelerate burned wound healing.