应用蛋白芯片技术从烧伤大鼠早期血清中快速筛选低分子量差异蛋白

The rapid detection of low molecular mass proteins differentially expressed in the early serum of burned rat using proteinchip technology

目的 筛选正常大鼠血清与烧伤大鼠早期血清中差异表达的低分子量蛋白,为进一步解释烧伤血清生物效应奠定基础。方法 利用SELDI(surfacedenhancedlaserdesorption ionization)蛋白质芯片技术对烧伤大鼠早期血清差异蛋白进行筛选,通过相应软件结合生物信息学方法进行初步分析。结果 与正常血清比较,烧伤后12h与2 4h血清与之分别存在11个和3 1个差异蛋白峰,烧伤后12h与2 4h血清之间存在15个差异蛋白峰,并发现一些呈现一定的正常与烧伤、烧伤后不同时相点规律性变化的蛋白质。结论 正常与烧伤、烧伤后不同时相点血清中低分子量蛋白存在明显差异,这种差异蛋白可能与烧伤病理生理和烧伤后血清生物效应密切相关。

Objective To detect differentially expressed low mass proteins in the serum between normal and burned rats, and lay a foundation to further explanation of the biological effect of the burn serum. Methods The differentially expressed low mass proteins were detected by SELDI proteinchip technology, and primarily analyzed by related software as well as bioinformatic method. Results As compared to the normal rat serum, the serum of postburn 12 h and 24 h had 11 and 31 differentially expressed protein peaks respectively. There are 15 differentially expressed protein peaks between the serum of postburn 12 h and 24 h. Meanwhile, a series of protein peaks were found, which changed depending on the degree of burn injury and the time of postburn. Conclusion There are obvious differentially expressed low mass proteins in the sera between normal and burned rats, which maybe have close relationship with the burn pathophysiology and the biological effect of burn serum.