摘要
将怀山药乙醇提取物采用溶剂萃取的方法,分成极性不同的五个部分,并首次用DPPH(2,2diphenyl1picrylhydrazyl)方法测定各部分的抗自由基活性,发现乙酸乙酯萃取部分活性最强,氯仿萃取部分次之,再其次是正丁醇和水溶性部分。乙酸乙酯和氯仿萃取部分较强的抗自由基活性主要归因于其中所含的多酚类成分。同时利用薄层层析(TLC)、紫外光谱(UV)、13C核磁共振(NMR)技术及显色反应对酚性成分进行了定性检验,并用FolinDenis法测定了各萃取部位中酚性成分含量,发现抗自由基活性与萃取物中多酚性成分含量有一定的相关性。因而在评价怀山药质量时,其中所含的酚性成分不应忽视。
The ethanol extracts of the crude drug Dioscorea opposita thumb(Huai Shanyao) were divided into five fractions by liquid-liquid partitions by the increasing polarity. The study on DPPH free radical scavenging activities showed that the ethyl acetate fraction has the strongest scavenging activity to DPPH free radicals.The next is chloroform fraction,followed by n-butanol and aqueous fraction. The stronger scavenging activity to DPPH free radicals of the ethyl acetate and chloroform fractions should be mainly due to the polyphenolics contained. The presence of phenolic compounds in the extracts was also analyzed qualitatively by TLC, 13C NMR,color reaction and quantitatively by spectrophotometric method(Folin-Denis).
出处
《天然产物研究与开发》
CAS
CSCD
2005年第3期272-274,共3页
Natural Product Research and Development
基金
CommitteeofScienceandTechnologyofHenanProvince(No.0123031000)
MinistryofScienceandTechnologyofthePeoplesRepublicofChina(999290106)
HenanInnovationProjectForUniversityProminentResearchTalents(2004KYCX018)
