烫伤大鼠休克期红细胞膜阴离子通透性的异常

erythrocyte membrane during shock stage in burned rats

我们采用NO_2^-、Cl^-快速检测法测定了25％TBSAⅢ度烫伤大鼠红细胞膜对阴离子的通透特性,并用红细胞膜阴离子运输专一性抑制剂DIDS来考察膜带3蛋白的功能状态。结果提示:烧伤休克期红细胞膜带3蛋白受损是导致膜对阴离子通透性异常的重要原因,且这种异常和体外热损伤红细胞膜阴离子通透性的变化趋势不一致,说明休克期的体液因素和能量代谢障碍可能参与和加重了这种损伤的发生和发展。

The anion transport property is the one of the most important functions of the erythrocyte. In this paper a new method is used to measure the NO_2^- and Cl^-ions transport across the erythrocyte membrane in burned rats. It was found that the NO_2^- and Cl^- trans- port rate across erythrocyte membrane became slower during shock stage in burned rats, be- ing contrary to the results found in heat--treated erythrocytes in vitro. By using a specific inhibitor of anion transport, DIDS, it was shown that the abnormality of NO_2^- transport across the erythrocyte membrane in burned rats is due probably to a damage of extracellular domain of band 3 protein. The authors consider that the influence of membranotoxic factors and the disturbance of energy metabolism during burn shock stage are accounted for all the noticed alterations.