严重烫伤后腹腔巨噬细胞游离钙及抗原提呈功能的实验研究

Experimental study on cytoplasmic free calcium and antigen presentation function of peritoneal macrophage following severe scald

我们对严重烫伤小鼠腹腔巨噬细胞内游离钙PM(?)[Ca^(2+)]和抗原提呈(AP)功能进行了动态观察,结果显示:体外未受刺激的PM(?)[Ca^(2+)]在烫伤后12、24、72h均显著升高。这证明烫伤后早期PM(?)在体内就已被激活。加A23187刺激的PM(?)[Ca^(2+)]在伤后虽无显著升高,但也提示PM(?)处于高度活化,甚至过度活化的状态。严重烫伤后可使PM(?)的AP能力明显低下,其不仅发生早,而且持续时间较久,可能已成为伤后特异性免疫功能受抑制的主导因素。M(?)体内活性的改变则可能是烧伤后M(?)免疫功能异常的基础。

A dynamic observation of cytoplasmic free calcium concentration (PM [Ca^(2+)]i) and antigen presentation (AP) function was made in severely scalded mice. The results showed that [Ca^(2+)] i of unstimulated PMφ in vitro increased significandy at 12, 24 and 72h postburn (PB), and the [Ca^(2+)] i of PM stimulated by A23187 in vitro was also increased but not statistically significant at the same time points. These results suggested that PM was activated in vivo, even in a highly or over activated state. Severe scald caused the obvious depression of AP function of PM. This phenomenon, not only appearing very early but also persisting for a long time, might become the key factor of suppression of spe- cific immune function PB. The changes in M activity in vivo might be the basic event of the M dysfunction after severe burns.