蒸汽吸入性损伤犬支气管肺泡灌洗液的观察

Study of bronchoalveolar lavage fluid harvested fromthe dogs with steam inhalation injury

为了探讨吸入性损伤后支气管肺泡灌洗液检测的意义,选用12条本地健康杂种犬进行自身对照试验。采用蒸汽致伤造成重度吸入性损伤,在伤后1小时和5小时观察支气管肺泡灌洗液(BALF)中细胞成份、总蛋白含量、丙二醛(MDA)含量、超氧化物歧化酶(SOD)活性和血管紧张素转换酶(ACE)活性的变化。结果表明,吸入性损伤后肺内大量炎性细胞渗出,脂质过氧化反应水平增高和肺毛细血管内皮细胞损伤。

The significances of analyzing bronchoalveolar lavage fluid (BALF) were studied using a steam inhalation injury model.Bronchoalveolar lavage was carried out at 1 and 5h after steam inhalation injury.The parameters investigated included celluar analysis, the total protein content,malondialdehyde (MDA) content,the activity of superoxide dismu- tase (SOD),and the angiotensin converting enzyme (ACE) in the BALF,and pathologic examination of lungs.The study indicated that some informations of the pathophysiological changes in the lungs after inhalation injury could be acquired by the BALF analysis.