50%Ⅲ度犬烧伤延迟补液后血浆SOD活性及MDA含量的变化

Changes in the contents of plasma SOD and MDA in 50% Ⅲ° burn dogs after delayed fluid replacement

采用50%Ⅲ度犬烧伤延迟补液模型,以黄嘌呤氧化酶法观察了血浆 SOD 变化,并以硫代巴比妥酸法测定了血浆 MDA 含量。结果表明:伤后血浆总-SOD 和 CuZn-SOD 活性迅速下降,且连续观察5天均明显低于伤前,而 Mn-SOD 变化不明显;血浆 MDA 伤后24 h 内明显高于伤前,48～72 h 降至伤前值,72h 后出现再次增高。结果提示,延迟输液将造成组织进一步损害,氧自由基产生增多,这可能是导致 MOF 发生的重要原因之一。

The plasma SOD and MDA content were determined in 50%Ⅲ° burn dogs with delayed fluid replacement.The results showed that the activity of plasma total SOD and CuZn-SOD rapidly declined and were obviously lower during the first 5 days postburn than preburn values,while the content of Mn-SOD did not show significant change.The plasma MDA was evidently elevated in 24 hour but returned to the normal level in 48～72 hours after burn,however it distinctly rose again after 72 hours post- burn.The results suggest that delayed fluid replacement induced generation of free radi- cals after burn injury,with the result of the occurrence of lipid peroxidation.