摘要
采用大鼠30%Ⅲ度烫伤模型,应用光镜、电镜、3β-羟甾脱氢酶(3β-HSD)组织化学染色及其相对活性的测定和血清睾酮及黄体生成素(LH)浓度的检测,分不同时相对严重烫伤后30天内大鼠睾丸间质细胞的改变进行了动态观察。结果表明,烫伤后睾丸间质细胞有不同程度的变性、坏死;间质细胞内3β-HSD 活性迅速降低,伤后30天仍保持较低水平;血清睾酮水平迅速下降,并持续维持较低水平,伤后30天仍不回升,而血清 LH 浓度无明显改变。提示烫伤后血清睾酮下降的机理可能与睾丸间质细胞的受损及糖皮质激素升高有关。
The changes in Leydig cells of testes were studied in rats inflicted with 30% third degree scald.Light and electron microscopy,enzyme histochemistry Of 3β-hy- droxy steroid dehydrogenase(3β-HSD)determination of the relative activity of 3β-HSD, and assay of serum testosterone and luteinizing hormone(LH)were dynamically made for 30 days.It was found that there was various degree of degeneration and necrosis of Leydig cells.The activity of 3β-HSD in Leydig cells was rapidly reduced and remained at a relatively low level on the 30 th day after scald.The serum level of testosterone was decreased rapidly and remainedat a comparatively low level on the 30th day after scald. No significant changes in serum luteinizing hormone were observed.The results suggeste that decreased in serum testosterone after scald might be the result of damage of the Leydig cells and increase in glucocorticoids.
关键词
烧伤
睾丸间质细胞
羟甾脱氢酶
睾酮
Burn
Testis Leydig cell
3 β-bydroxy steroid dehydrogenase
Testosterone
