右旋糖酐-甲基丙烯酸缩水甘油酯载BMP_2凝胶微球的制备与评价

Preparation and Evaluation of BMP_2 Carrying Gel Microspheres by Dextran-glycidyl Methacrylate

目的:探讨利用右旋糖酐-甲基丙烯酸缩水甘油酯(DEX-GMA)制备载骨形态发生蛋白2(BMP2)凝胶微球的可行性和制备工艺,并初步探讨其载药、释药特性。方法:以反应温度、DEX-GMA和乳化剂Span-80的加入量和搅拌速率等为考察因素进行正交试验,筛选BMP2-DEX-GMA凝胶微球的最佳制备工艺并对成品性能进行初步检测。结果:经悬浮聚合法可以制备BMP2-DEX-GMA凝胶微球,优选出的制备工艺条件为反应温度30℃、DEX-GMA用量0.6%、Span-80用量0.06%、搅拌速率300r/min,由此制备的BMP2-DEX-GMA凝胶微球形态良好,粒径在20μm～50μm之间,包封率为(78.52±4.34)%,载药量为(10.68±1.34)%,溶胀率为85.9%,稳定性和再分散性良好。结论:该凝胶微球载药系统制备工艺简单,载药量大,可以负载具有生物活性的药物。

OBJRCTIVE:To study the feasibility and the preparation of bone morphogenetic protein-2(BMP 2 )carrying gel microspheres by dextran-glycidyl methacrylate(DEX-GMA)and to make an initial study on the drug-loading and drug releasing function of gel.METHODS:The orthogonal test was conducted with the reaction temperature,the addition of DEX-GMA and Span-80,the stirring speed and so on as investigation factors,the best preparation technics of BMP 2 -DEX-GMA gel microspheres was optimized and the finished products of which were given an initial determination.RE-SULTS:The BMP 2 -DEX-GMA gel microspheres preparation could be made by suspension polymerization,the optimized preparation technics including the reaction temperature was30℃,the addition of DEX-GMA and Span-80were0.6%and0.06%respectively,the stirring speed was300r/min,the BMP 2 -DEX-GMA gel microspheres from the above formulation take good shapes with the particle diameter at20μm～50μm,the envelopment ratio was(78.52±4.34)%,the quantity of drug-loading was(10.68±1.34)%,and the swelling ratio was85.9%,which has a good stability and redispersibility.CON-CLUSION:The preparation technics of gel microspheres drug-loading system is simple and the loading dosage is high,which can be used as a bioactive drugs carrier.