脂氧合酶、脱落酸与茉莉酸在合作杨损伤信号传递中的相互关系

Relationship between Lipoxygenase and ABA and JA in Wounded Signal Transduction of Heathy Populus Seedlings

以合作杨为实验材料,检测了人为机械损伤后2、4、8、12、24、48h损伤叶片与邻近健康植株叶片内脂氧合酶(Lipoxygenase,Lox)活性、脱落酸(ABA)、茉莉酸(JA)含量的变化情况。结果显示:(1)人为损伤不但引起损伤植株体内ABA、JA含量及Lox活性的升高,而且健康植株体内该三种物质的变化与之有相同的升高趋势,表明植物体受伤后,在体内迅速合成信号物质发生防御反应,这种防御反应不仅在受损伤的植株体内发挥作用,还可以释放某种挥发性物质并通过空气传递到邻近健康植株,诱导邻近植株产生抗性;(2)在ABA、JA积累的同时,Lox的活性也升高且趋势一致,Lox与ABA、JA的合成有密切关系。

Using the poplar as materials, the authors examined and compared the activity of lipoxygenase (Lox) and the dynamic level of ABA and JA in different leaves: the mechanical damaged leaves and the leaves of the neighboring healthy plant after being damaged. The result showed that : (1) the mechanical wounding enhanced the activity of Lox and induced the concentration of ABA and JA increasing in the leaves. It could be concluded that mechanical wounding could induce the defensive of the damaged plant. Furthermore, there might exist some airborne signals, which transferred the wounding message to neighboring plants, and induced defense reaction of the neighboring healthy plants. (2) Lox activity and the contents of ABA and JA increased with similar trend along with the mechanical wounding, indicating that Lox maybe act to the key process of the biosythesis to ABA and JA.