高转移人肺巨细胞癌细胞67－KD层粘连蛋白受体基因表达

THE EXPRESSION OF 67 KD LAMININ RECEPTOR (LN R)GENE IN PG TUMOR CELLS WITH HIGH METASTATIC POTENIAL

在逆转录ＰＣＲ特异扩增６７一ＫＤ层粘连蛋白受体（简称ＬＮ—Ｒ）ｃＤＮＡ片段并制备成探针的基础上，观察了高转移人肺巨细胞癌（简称ＰＧ）细胞和低转移人肺腺癌（简称ＰＡａ）细胞６７—ＫＤＬＮ—ＲｍＲＮＡ表达水平。结果表明，两种细胞存在大小相同的特异转录体，约１．７ｋｂ左右，高转移ＰＧ瘤细胞比低转移ＰＡａ瘤细胞表达较高水平的６７—ＫＤＬＮ—ＲｍＲＮＡ，同时ＰＧ瘤细胞存在明显的该基因扩增；不同剂量６７—ＫＤＬＮ—Ｒ单克隆抗体（５０μｇ／ｍｌ、１００μｇ／ｍｌ和４００μｇ／ｍｌ）处理ＰＧ瘤细胞４８小时，可见１００μｇ／ｍｌ以上剂量组细胞６７—ＫＤＬＮ—ＲｍＲＮＡ表达水平明显下降，表现为剂量依赖性抑制。这些结果提示，６７—ＫＤＬＮ—Ｒ在高转移ＰＧ瘤细胞转移过程中可能起有重要作用。

Abstract The expression level of 6 KD LN R mRNA was observed in high metastatic PG tumor cells and low -metastatic PAatumor cells based on the cDNA fragments of 67-KD LN -R amplified by PCR technique and the specific cDNS probe prepared by random, primer labeling method. Results showed that the transcripts were homologous in size, about 1.7kb,in PG and PAa tumor cells . The 67 KD LN R mRNA level was higher in PG than in PAa tumor cells, and gene amplification was also more markedin PG tumor cells . After treated with 67-KD LN-Rmonocolnioalantibody (50,100 and 400μg/ml)for 48 hours,LN-R mRNA of PG tumor cells de-creased significantly.It is suggested that 67 KD LN R may play important roles in metastatic pro-cesses of PG tumor cells.