摘要
在逆转录PCR特异扩增67一KD层粘连蛋白受体(简称LN—R)cDNA片段并制备成探针的基础上,观察了高转移人肺巨细胞癌(简称PG)细胞和低转移人肺腺癌(简称PAa)细胞67—KDLN—RmRNA表达水平。结果表明,两种细胞存在大小相同的特异转录体,约1.7kb左右,高转移PG瘤细胞比低转移PAa瘤细胞表达较高水平的67—KDLN—RmRNA,同时PG瘤细胞存在明显的该基因扩增;不同剂量67—KDLN—R单克隆抗体(50μg/ml、100μg/ml和400μg/ml)处理PG瘤细胞48小时,可见100μg/ml以上剂量组细胞67—KDLN—RmRNA表达水平明显下降,表现为剂量依赖性抑制。这些结果提示,67—KDLN—R在高转移PG瘤细胞转移过程中可能起有重要作用。
Abstract The expression
level of 6
KD LN R mRNA was observed in high metastatic PG tumor cells and low -metastatic PAatumor
cells based on the cDNA fragments of 67-KD LN -R amplified by PCR technique and the specific
cDNS probe prepared by random, primer labeling method. Results showed that the transcripts
were homologous in size, about 1.7kb,in PG and PAa tumor cells . The 67 KD LN R mRNA level
was higher in PG than in PAa tumor cells, and gene amplification was also more markedin PG
tumor cells . After treated with 67-KD LN-Rmonocolnioalantibody (50,100 and 400μg/ml)for 48
hours,LN-R mRNA of PG tumor cells de-creased significantly.It is suggested that 67 KD LN R
may play important roles in metastatic pro-cesses of PG tumor cells.
出处
《中华肿瘤杂志》
CAS
CSCD
北大核心
1994年第6期403-406,共4页
Chinese Journal of Oncology
基金
国家"八五"攻关项目课题