KiSS-1基因及其蛋白在人胰腺癌组织中表达及其与侵袭和转移的关系(英文)

Expression of KiSS-1 in human pancreatic cancer and relationship with their invasion and metastasis

目的研究胰腺癌组织中KiSS-1表达及其与侵袭和转移的关系。方法应用RT-PCR和westernblot法检测KiSS-1mRNA及其蛋白肽metastin在37例胰腺癌及27例转移灶和9例正常胰腺组织中的表达情况。结果在胰腺癌组织中KiSS-1基因及其蛋白表达水平显著低于正常胰腺组织(P<0.01,P<0.01)。KiSS-1mRNA及其蛋白的表达与性别、年龄、肿瘤大小及组织学分级无关,与临床分期、有无转移和神经侵犯密切相关。在III、IV期胰腺癌组织中KiSS-1基因及蛋白的表达分别为(0.135±0.121)和(9.23±2.74)μg/100μg总蛋白,明显低于I、II期(P<0.05,P<0.05)。KiSS-1基因及其蛋白在无转移、有转移的胰腺癌原发灶和转移灶组织中表达水平呈降低趋势,转移灶中的表达水平最低。有神经侵犯的胰腺癌原发灶KiSS-1基因及蛋白表达显著低于无神经侵犯的胰腺癌原发灶(P<0.01,P<0.01),其表达与有无神经侵犯相关系数分别为Kendallτ-b=0.597(P=0.003)和Kendallτ-b=0.438(P=0.007)。结论KiSS-1基因转录和表达降低与胰腺癌侵袭和转移密切相关,可能参与胰腺癌转移的调控。

[Objective] To investigate the expression of KiSS-1 in human pancreatic cancer in relation to the clinical significance. [Methods] RT-PCR analysis and western blot analysis were used to detect the expression level of KiSS-1mRNA and its protein peptide-metastin in tissues of 37 cases of patients with pancreatic cancer, 27 cases of their metastatic site and 9 cases of normal pancreatic tissues. [Results] The expression of KiSS-1 in pancreatic tissues were significantly lower than those in normal (P <0.01, P <0.01). The expression of KiSS-1 had no correlation with sex, age, size of tumor and histological grade. However, the expression of KiSS-1 was related to the clinical stage, metastasis and nerves invasion significantly. The expression of KiSS-1mRNA and metastin were (0.135±0.121) and (9.23±2.74) μg /100 μg total protein separately in stage III and IV, which were significant lower than those in stage I and II (P <0.05, P <0.05). It showed a decreased tendency in pancreatic cancer without metastasis, with metastasis and metastatic site, which was lowest in metastatic site. The expression of KiSS-1 in tumor with nerves invasion were significantly lower than those without nerves invasion (P <0.01, P <0.01), which correlated coefficient (Kendall τ-b) were 0.597(P =0.003) and 0.438 (P =0.007). [Conclusion] The expression of KiSS-1 has close relation to invasion and metastasis of human pancreatic cancer, and may be involved in regulation of this process.