FasL系统在妊高征胎盘中的表达

Placental expression of Fas and FasL in patients with pregnancy induced hypertension

目的检测凋亡诱导蛋白Fas及其配体FasL在妊娠高血压综合征(PIH)胎盘中的表达,探讨FasL系统在PIH发病中的作用。方法应用免疫组化法检测10例正常足月孕妇及10例重度PIH孕妇胎盘组织中Fas、FasL的表达,并应用BI-2000医学图像分析系统对其表达进行定量分析。结果两组胎盘绒毛滋养细胞均有Fas、FasL表达,其表达量比较,差异有显著性。PIH组绒毛滋养细胞FasL表达的平均灰度、染色面积比分别为(93·390±7·600)、(0·060±0·019),明显低于正常妊娠组(73·104±7·788)、(0·121±0·019),两组间差异有显著意义(P<0·05);PIH组胎盘绒毛滋养细胞Fas表达的平均灰度、染色面积比分别为(75·212±4·688)、(0·120±0·009),明显高于正常妊娠组(97·445±6·019)、(0·057±0·011),两组差异有极显著意义(P<0·01)。结论PIH时胎盘中FasL表达下调,Fas表达增加。FasL系统表达异常可能参与PIH的发病。

Objective To explore the function of placental trophoblast cell apoptosis on the pathogenetic mechanism of pregnancy induced hypertension (PIH). Methods The expression of Fas and FasL in placenta trophoblasts in 10 severe PIH patients (PIH group) and in 10 cases of normal pregnancies (control group)were directly observed using immunohistochemistry method, the quantity of Fas and FasL was determined using BI-2000. Results The strength and the scale ratio of FasL expression in the PIH group〔(93.390±7.600) and (0.060±0.019)〕,were significantly lower than those of control group〔(73.104±7.788)and (0.121±0.019),P<0.05〕;but the strength and the scale ratio of Fas expression in the PIH group〔(75.212±4.688) and (0.120±0.009)〕, were significantly higher than those of control group〔(97.445±6.019) and (0.057±0.011),P<0.01〕. Conclusion The expression of FasL is lower and that of Fas is higher in placenta in patients with PIH,which results in the apoptosis of placental trophoblast cells and thus contributes to the pathogenesis of PIH.