电针对大鼠高血压性脑出血血肿周围组织NF-κB TNF-α表达的影响

Effect of Electroacupuncture on NF-KB and TNF-αin the Brain Tissue Around Hematoma of the Rats with Hypertensive Cerebral Hemorrhage

目的:观察电针水沟穴对大鼠高血压性脑出血血肿周围组织NF-kB、TNF-α表达的影响,初步探讨二者在脑出血发生中的意义及电针对其的干预作用。方法:在大鼠自发性高血压基础上,以胶原酶加肝素脑内注射诱发脑出血的方法,建立大鼠高血压性脑出血模型,运用免疫组织化学技术分析各组NF-kB、TNF-α的表达水平。结果:模型组大鼠高血压性脑出血血肿周围组织NF-kB、TNF-α表达水平较正常对照组大鼠显著增强,电针治疗后其表达明显下调。结论:电针可有效抑制高血压性脑出血大鼠脑组织NF-kB、TNF-α的表达水平,进而减轻高血压性脑出血后脑免疫炎性损伤,这可能是电针治疗高血压性脑出血的重要分子机制之一。

Objective: To observe the effect of electroacupuncture on NF-kB and TNF-α in the brain tissue around hematoma of the rats with hypertensive cerebral hemorrhage in order to study the function of NF-kB and TNF-α in cerebral hemorrhage and the intervention of electroacupuncture on them. Methods:The hypertensive cerebral hemorrhage rat model was evoked by injecting collagenase and heparin into the brain of the spontaneously hypertensive rat. The expressions of NF-kB and TNF-α were detected by immunohistochemical methods. Results: The expressions of NF-kB and TNF-α in the brain tissue around hematoma of the rats in hypertensive cerebral hemorrhage model group were significantly up - regulated compared with those in normal control group, while after electroacupuncture treatment they were significantly down-regulated. Conclusion: Electroacupuncture may effectively inhibit the expressions of NF-kB and TNF-α in brain tissue of the rats with hypertensive cerebral hemorrhage to relieve the lesion from the immunologic inflammation after hypertensive cerebral hemorrhage. This may be one of the importanc molecular mechanisms of electroacupuncture on hypertensive cerebral hemorrhage.