基因重组干扰素α-2b脂质体的药代动力学研究

Study on the Pharmacokinetics of Recombinant Interferon α-2b Liposome

研究注射用干扰素α2b脂质体在大鼠体内的药代动力学及小鼠组织分布、排泄。运用同位素示踪法结合SDSPAGE,测定静脉注射后大鼠血液中原型药物浓度并做其胆汁排泄试验;用小鼠进行组织分布试验和尿粪排泄试验。结果:符合一级消除动力学的二房室模型,干扰素α2b的t1/2β为0.69h,脂质体3个剂量的t1/2β分别为3.36h、3.02h、2.34h,干扰素α2b脂质体在大多组织中分布增加,肝脾中尤其明显。脂质体能够显著延长干扰素α2b在血液及组织中半衰期,提高靶向性。

Pharmacokinetics of IFN α-2b and IFN α-2b liposome in rats was determined and tissue distribution and excretion were also studied. The pharmacokinetic process was studied after iv inecting different dosages of IFN α-2b and liposome by  125 I labeling and SDS-PAGE technique. The concentration-time curves of the two proteins were fitted to two-compartment model. The t 1/2β of INF α-2b was 0.69 h, while IFN α-2b liposome was 3.36 h, 3.02 h and 2.34 h. IFN α-2b liposome was increased in most of tissues especially in liver and spleen. Liposome can lengthen the half life of IFN α-2b in serum and tissues.