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4-氨基吡啶对皮层神经元细胞内游离钙的影响

4-Aminopyridine-Induced Increases in Intracellular Free Ca^(2+) in Cultured Mouse Cortical Neurons
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摘要 目的研究4-氨基吡啶(4-AP)对体外培养的皮层神经元细胞内游离钙浓度([Ca2+]i)的影响,了解4-AP的药理学作用机制.方法荧光探针Fluo-3-AM标记体外培养的皮层神经细胞内游离钙后,用共聚焦显微镜观察记录4-AP及L型谷氨酸对小鼠原代培养的皮层神经元[Ca2+]i的影响.结果4-AP与谷氨酸均能提高[Ca2+]i,两者峰值与持续时间存在差异,共同作用于细胞时的上升曲线与单用谷氨酸时相仿.结论4-AP的药理作用机制可能与提高神经细胞[Ca2+]i有关,其中机制与兴奋性氨基酸的[Ca2+]i升高作用可能不同. ObjectiveTo analyze the effect of 4-aminopyridine (4-AP) on free intracellular calcium concentration (i) in cultured mouse cortical neurons and explore the possible pharmacological mechanism. MethodsApplying intracellular calcium probe Fluo-3-AM to indicate the changes of i; Using confocal microscopy to observe the calcium signal and the effect of 4-AP and L-glutamate on it. Results4-AP and glutamate could increase i significantly, whereas the calcium response curve to both reagents was characterized for each other. The i curve in response to simultaneous action of 4-AP and glutamate was similar to the effect of only glutamate. ConclusionThe pharmacological effects of 4-AP may be related with the complex change of calcium homeostasis, the underlying mechanism of which are possibly different from that of glutamate-induced change.
作者 苏涛 陈盛强 邓维意 姜涛 丛文东 廖卫平
机构地区 广州医学院第二附属医院神经科学研究所
出处 《现代临床医学生物工程学杂志》 2005年第2期94-96,共3页 Journal of Modern Clinical Medical Bioengineering
关键词 4-氨基吡啶 细胞内游离钙离子浓度 谷氨酸 共聚焦技术 皮层神经细胞 4-Aminopyridine, i, Glutamate, Confocal Microscopy, Cortical neurons culture
分类号 R96 [医药卫生—药理学]
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参考文献9

  • 1Andreani A, Leoni A, Locatelli A, et al. 4-Aminopyridine derivatives with antiamnesic activity[J].Eur J Med Chem,2000,35(1):77.
  • 2Smith KJ, Felts PA, John GR. Effects of 4-aminopyridine on demyelinated axons, synapses and muscle tension[J].Brain,2000,123(1):171.
  • 3Campbell DL, Qu Y, Rasmusson RL, et al. The calcium-independent transient outward potassium current in isolated ferret right ventricular myocytes. Ⅱ. Closed state reverse use-dependent block by 4-aminopyridine[J].J Gen Physiol,1993,101(4):603.
  • 4Lipskaia L, Lompre AM. Alteration in temporal kinetics of Ca^2+ signaling and control of growth and proliferation[J].Biol Cell,2004,96(1):55.
  • 5Grimaldi M, Atzori M, Ray P, Alkon DL. Mobilization of calcium from intracellular stores, potentiation of neurotransmitter-induced calcium transients, and capacitative calcium entry by 4-aminopyridine[J]. J Neurosci,2001,21(9):3135.
  • 6Basavappa S, Romano-Silva MA, Mangel AW, et al. Inhibition of K^+ channel activity by 4-AP stimulates N-type Ca^2+ channels in CHP-100 cells[J]. Neuroreport,1994,5(10):1256.
  • 7Wang G, Ding S, Yunokuchi K. Glutamate-induced increases in intracellular Ca^2+ in cultured rat neocortical neurons[J]. Neuroreport,2002,13(8):1051.
  • 8胡波,孙圣刚,何立铭,张春光,童萼塘.谷氨酸诱发培养的大鼠星形胶质细胞内钙升高的机制[J].中国神经科学杂志,2004,20(1):24-28. 被引量：11
  • 9Spyker DA, Lynch C, Shabanowitz J, et al. Poisoning with 4-aminopyridine: report of three cases[J]. Clin Toxicol,1980,16(4):487.

二级参考文献19

  • 1[1]Carmignoto G. Reciprocal communication systems between astrocytes and neurones[J]. Prog Neurobiol, 2000,62(6) :561-581.
  • 2[2]Shelton MK, McCarthy KD. Mature hippocampal astrocytes exhibit functional metabotropic and ionotropic glutamate receptors in situ [J]. Glia, 1999,26(1):1-11.
  • 3[3]Sanzgiri RP, Araque A, Haydon PG. Prostaglandin E(2) stimulates glutamate receptor-dependent astrocyte neuromodulation in cultured hippocampal cell[J]. J Neurobiol, 1999,41 (2):221-229.
  • 4[4]Parpura V, Haydon PG. Physiological astrocytic calcium levels stimulate glutamate release to modulate adjacent neurons [J]. Proc Natl Acad Sci USA, 2000,97(15) :8629-8634.
  • 5[5]Mc Carthy K, Devellis J. Preparation of separate astroglial and Ol-igodendroglial cultures from raty cerebral tissue[J]. J Cell Bio,1980,85 (3) :890-892.
  • 6[6]Chen LY, Zhou SB, Lou XL, et al. Different stimulatory opioid effects on intracellular Ca2+ in SH-SY5Y cells[J]. Brain Res,2000,882:256-265.
  • 7[7]Nishizawa. Glutamate release and neuronal damage in ischemia [J]. Life Sci, 2001,69(4) :369-381.
  • 8[8]Koinig H, Vornik V, Rueda C, et al. Lubeluzole inhibits accumulation of extracellular glutamate in the hippocampus during transient global cerebral ischemia[J]. Brain Res, 2001,898(2) :297302.
  • 9[9]Chen Y, Vartiainen NE, Ying W, et al. Astrocytes protect neurons from nitric oxide toxicity by a glutathione-dependent mechanism[J]. J Neurochem, 2001,77(6) :1601-1610.
  • 10[10]Oliet SHR, Pict R, Poulain DA. Control of glutamate clearance and synaptic efficacy by glial coverage of neurons [J]. Science,2001,292 (5518) :923-926.

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现代临床医学生物工程学杂志
2005年 第2期

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