扇贝多肽对UVB损伤HaCaT细胞的抗氧化作用

Antioxidative effect of polypeptide from Chlamys farreri on HaCaT cells damaged by ultraviolet B

目的研究扇贝多肽(PCF)对UVB损伤HaCaT细胞的抗氧化作用。方法HaCaT细胞与PCF孵育1h后,接受UVB辐射,继续孵育18h后,采用酶化学法测定细胞超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSHPx)、过氧化氢酶(CAT)活性、以及测定其总抗氧化能力(TAOC)、丙二醛(MDA)水平。结果PCF能提高HaCaT细胞内抗氧化酶SOD,GSHPx,CAT活性,增强细胞总抗氧化能力并减少脂质过氧化产物MDA的产生。结论PCF能增加细胞内抗氧化酶活性,抑制脂质过氧化反应,具有抗氧化作用。

Objective To investigate the antioxidative effect of polypeptide from Chlamys farreri (PCF) on HaCaT cells damaged by ultraviolet B (UVB). Methods HaCaT cells model established by UVB irradiation were randomly divided into seven groups. After pretreatment with different concentrations of PCF for 1 h, the cells were irradiated by UVB at a dose of 30mJ/cm~ 2, and followed further incubatiou for 18 h. Enzyme activities including superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase (CAT) were determined by biochemical methods. Total antioxidative capacity (T-AOC) and malondialdehyde (MDA) were also determined. Results PCF could enhance the activities of SOD, GSH-Px and CAT; increased T-AOC and inhibited MDA formation. Conclusion These results indicated that PCF exhibited protective effects on HaCaT cells irradiated by UVB owing to its antioxidative action.